ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR THE ESTIMATION OF CANAGLIFLOZIN IN BULK AND FORMULATION BY RP- HPLC
Journal Title: International Journal of Pharmaceutical Sciences and Drug Research - Year 2018, Vol 10, Issue 3
Abstract
A simple and sensitive reverse phase high performance liquid chromatographic method was developed and successively validated for the estimation of Canagliflozin. In the new method, Canagliflozin separation was carried out by the nonpolar inertsil ODS-3 (250 × 4.6 mm, 5μ) column with a mobile phase composition of Ammonium acetate buffer (pH-4.5) and Acetonitrile in the ratio of 30:70% v/v. Canagliflozin was determined at 252 nm using UV detection and the compound was eluted at the retention time of 4.5 min. As per International Conference on Harmonization (ICH) guidelines, the method was validated and the parameters were precision, accuracy, linearity, limit of detection, limit of quantitation and robustness. The chromatographic method was accurate, linear, specific, precise and robust. The results of method concluded that the proposed RP-HPLC method is useful, convenient and reliable in regular analysis of Canagliflozin in bulk and its formulation.
Authors and Affiliations
Darshan Bhatt, Padmini Thatavarthi, B. Rajkamal
Keywords
Related Articles
- EP ID EP618402
- DOI 10.25004/IJPSDR.2018.100306
- Views 97
- Downloads 0
Identification of Phenyl Alanine Ammonia Lyase Gene Involved in the Synthesis of Anacardic Acid in Anacardium occidentale L.
Anacardic acids, a class of medicinally and industrially important phenolic compounds is found in a variety of dicotyledonous families chiefly in Cashew (Anacardium occidentale L). Phenylalanine ammonia-lyase (PAL) shows...
EXTRACTIVE SPECTROPHOTOMETRIC METHODS FOR THE DETERMINATION OF GABAPENTIN IN PHARMACEUTICAL DOSAGE FORMS
Two simple, rapid, sensitive, accurate, precise and economic spectrophotometric methods have been developed for the estimation of Gabapentin (GBP) in pharmaceutical formulations. During the course of study, it was observ...
FORMULATION, OPTIMIZATION AND IN VITRO EVALUATION OF RIFAMPICIN NANOEMULSIONS
The aim of the present investigation was to develop, optimize and evaluate nanoemulsion of rifampicin to improve the solubility, stability and oral bioavailability. Rifampicin nanoemulsions were prepared by o/w nanoemuls...
IMMUNOMODULATORY EFFECTS OF TINOSPORA CORDIFOLIA LOTION ON INTERLEUKIN-1, INTERLEUKIN-6 AND INTERLEUKIN-8 LEVELS IN SCABIES-INFECTED PEDIATRIC PATIENTS: A SINGLE BLIND, RANDOMIZED TRIAL
Scabies is a contagious, parasitic skin infestation caused by Sarcoptes scabiei mite, which has the ability to regulate the host’s inflammatory and immune responses. It is a serious community health problem in many less-...
FORMULATION, CHARACTERIZATION AND IN VITRO EVALUATION OF ENTACAPONE SOLID DISPERSIONS WITH LIPID CARRIERS BY USING SPRAY DRYING METHOD
In present study, immediate release solid dispersion formulation of Entacapone with various lipids was developed using spray drying method. Entacapone is indicated for the treatment of Parkinson’s disease (PD) as an adju...