ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR THE ESTIMATION OF TEMOZOLOMIDE IN PHOSPHATE BUFFER PH 2.0 AS A SOLVENT BY UV SPECTROSCOPY
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2014, Vol 5, Issue 1
Abstract
Temozolomide is an antineoplastic agent with activity against a broad spectrum of murine tumors. This compound is currently marketed for the treatment of patients with glioblastoma multiforme and anaplastic astrocytoma, which are serious and aggressive types of brain cancers. The present research work discusses the development and validation of a UV spectrophotometric method for temozolomide. Simple, accurate, precise and cost efficient spectrophotometric method has been developed for the estimation of temozolomide in bulk and capsule dosage form. The optimum conditions for the analysis of the drug were established. The maximum wavelength (λ max) was found to be 330 nm in Phosphate Buffer pH 2.0. The percentage recovery of temozolomide was found to be in range 98.47 – 100.4 %. Beers law was obeyed in the concentration range of 4-18 μg/ml. Calibration curves shows a linear relationship between the absorbance and concentration. The line equation y = 0.053x + 0.006 with r2 of 0.999 was obtained. Validation was performed as ICH guidelines for Linearity, accuracy, precision, LOD and LOQ. The proposed method may be suitable for the analysis of temozolomide in bulk and capsule formulation for quality control purposes.
Authors and Affiliations
B. Ishaq, Hindustan Ahad, Muneer Shaik , S. Parveen
Keywords
Related Articles
- EP ID EP146986
- DOI 10.7897/2230-8407.050104
- Views 58
- Downloads 0
EVALUATION OF CARDIO PROTECTIVE ACTIVITY OF ETHANOLIC EXTRACT OF ALSTONIA SCHOLARIS ON ISOPROTERENOL INDUCED MYOCARDIAL INFARCTION IN RATS
The present study was designed to evaluate the cardio protective potential of ethanolic extract of Alstonia scholaris Linn., (Family, Apocynaceae) is an evergreen tree with white, strongly perfumed flowers on isoproteren...
HEPATOPROTECTIVE EFFECT OF AQUEOUS AND N-HEXANE EXTRACT OF NIGELLA SATIVA IN PARACETAMOL (ACETAMINOPHEN) INDUCED LIVER DISEASES OF RATS: A HISTOPATHOLOGICAL EVALUATION
Acute over dose of paracetamol (acetaminophen) causes serious hepatic necrosis. So, this study was conducted to observe the hepatoprotective activity of aqueous and n-hexane extract of Nigella sativa in paracetamol induc...
PHYTOCHEMICALS ANALYSIS AND TLC FINGERPRINTING OF METHANOLIC EXTRACTS OF THREE MEDICINAL PLANTS
The present work is done on three medicinal plants (Enhydra fluctuans, Lecuas aspera and Dillinia indica) in order to investigate the presence of the various types of Phytoconstituents. The leaves of all three plants wer...
MOLECULAR PHYLOGENETIC STUDY OF PERIPLANETA FULIGINOSA FROM LAKSHADWEEP ISLANDS, INDIA USING CYTOCHROME OXIDASE SUBUNIT GENE SEQUENCE
Cockroaches are insects of the order Blattoidea, sometimes also called Blattaria. Cockroaches live in a wide range of environments around the world, having broad, flattened bodies and relatively small heads. They are gen...
STUDY ON EXTRACTS OF PARMELIA PERLATA ACH. FOR ITS ANTIMICROBIAL POTENTIAL AGAINST CERTAIN MICROORGANISMS
A significant increase in the trend of infectious diseases and alarming shoot up of resistance to existing antimicrobial agents demands a renewed effort to seek agents effective against pathogenic bacteria resistant to c...