ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR THE ESTIMATION OF TEMOZOLOMIDE IN PHOSPHATE BUFFER PH 2.0 AS A SOLVENT BY UV SPECTROSCOPY
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2014, Vol 5, Issue 1
Abstract
Temozolomide is an antineoplastic agent with activity against a broad spectrum of murine tumors. This compound is currently marketed for the treatment of patients with glioblastoma multiforme and anaplastic astrocytoma, which are serious and aggressive types of brain cancers. The present research work discusses the development and validation of a UV spectrophotometric method for temozolomide. Simple, accurate, precise and cost efficient spectrophotometric method has been developed for the estimation of temozolomide in bulk and capsule dosage form. The optimum conditions for the analysis of the drug were established. The maximum wavelength (λ max) was found to be 330 nm in Phosphate Buffer pH 2.0. The percentage recovery of temozolomide was found to be in range 98.47 – 100.4 %. Beers law was obeyed in the concentration range of 4-18 μg/ml. Calibration curves shows a linear relationship between the absorbance and concentration. The line equation y = 0.053x + 0.006 with r2 of 0.999 was obtained. Validation was performed as ICH guidelines for Linearity, accuracy, precision, LOD and LOQ. The proposed method may be suitable for the analysis of temozolomide in bulk and capsule formulation for quality control purposes.
Authors and Affiliations
B. Ishaq, Hindustan Ahad, Muneer Shaik , S. Parveen
Keywords
Related Articles
- EP ID EP146986
- DOI 10.7897/2230-8407.050104
- Views 111
- Downloads 0
METHOD DEVELOPMENT AND VALIDATION FOR THE ESTIMATION OF ATENOLOL IN TABLET DOSAGE FORM BY RP-HPLC METHOD
A simple, rapid, precise and accurate reverse phase high performance liquid chromatography has been developed and validated for the estimation of Atenolol in tablet formulations. Separation was carried on Waters e 2695 I...
TOXICITY ASSESSMENT OF MUCUNA PRURIENS LINN. SEEDS
Plants have been used as medicine for the treatment of diseases for thousands of years. These herbal remedies, although natural, can cause some serious damaging effects on the vital organs of the body due to inadequacy i...
COMPARATIVE SEM STUDY ON TETRACYCLINE HYDROCHLORIDE ROOT CONDITIONING: THE EFFECTS OF DIFFERENT CONCENTRATIONS AND APPLICATION TIMES
Smear layer removal and collagen fiber exposure may improve periodontal treatment and regeneration. This in vitro study assessed smear layer removal and morphological changes (collagen fiber exposure) after tetracycline...
FORMULATION AND EVALUATION OF ORODISPERSIBLE TABLETS OF DIAZEPAM USING DIFFERENT SUPERDISINTEGRANTS
Diazepam orodispersible tablets were prepared to achieve quick onset of action and for maximum bioavailability. The purpose of the present research was to compare the effect of different superdisintegrants on the mouth d...
NOVEL ANALYTICAL METHOD FOR IMPROVEMENT OF AQUEOUS SOLUBILITY OF CANDESARTAN CILEXETIL USING CO-SOLVENCY APPROACH
The present study was aimed at improving the aqueous solubility of candesartan cilexetil, a poorly water soluble drug, using poly ethylene glycol (PEG) 400 as co-solvent. It was observed that use of 5 % PEG 400 as co-sol...