Analytical method development and validation of Glimepiride in bulk and tablet dosage form using UV Spectrophotometer
Journal Title: International Journal of Pharmacy and Analytical Research - Year 2013, Vol 2, Issue 4
Abstract
The main objective of the study is to develop and validate an analytical method for quantitative determination of Glimepiride in bulk and tablet dosage form using UV-Visible Spectroscopy. The Glimepiride shows maximum absorption at 231nm and obeys Beer’s law in the range of 5-10µg/ml.For the method development we have selected a perfect solvent system using solvent such as NaOH. Calibration curve has been plotted. The assay should be carried and percentage recovery needs to be calculated. For the validation of the analytical method developed is carried out by determining parameters like Linearity, range, LOD, LOQ, Accuracy, Precision, Ruggedness.The calibration plot did not deviate from linearity because of its low intercept value, the LOD and LOQ values were found to be 25.93µg/ml and 86.44µg/ml respectively which shows the sensitivity of the method. The ruggedness is found to be less than 2%. The percentage recovery was assessed using 3 different solutions of 8.0, 10.0, 12.0 µg/ml and the results obtained were 98, 101.2, and 102% respectively. The developed method was applied to the quantification of Glimepiride in tablets available in local market. It can be seen that the results obtained by proposed method was very much similar to that of established methods.
Authors and Affiliations
V Asha Ranjani
Keywords
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