Analytical Method Development and Validation of Metformin Hydrochloride and Benfotiamine in Bulk and Marketed Formulations

Journal Title: Saudi Journal of Medical and Pharmaceutical Sciences - Year 2017, Vol 3, Issue 12

Abstract

Abstract:In the present work, two simple and sensitive UV spectrophotometric methods have been developed for the quantitative estimation of Metformin Hydrochloride and Benfotiamine in combination using bulk and pharmaceutical dosage forms. Method A is Simultaneous equation method which involves measurement of absorbance at selected wavelength and solving the simultaneous equation to calculate the amount of drug present. Distilled water was used as the solvent for the analysis of both drugs. Two wavelengths 230 and 246 nm were selected for the estimation of Metformin Hydrochloride and Benfotiamine. LOD for Metformin Hydrochloride and Benfotiamine were found to be 0.03µg/ml and 0.27µg/ml respectively. Method validation was done as per ICH guidelines. Method B is Absorbance ratio method which involves, formation of Q-Absorbance equation at 239 nm (isoabsorptive point) and 246 nm (λmax of Benfotiamine) in distilled water. The Linearity lies between 2-16 µg/ml for Metformin Hydrochloride and 2-18 µg/ml for Benfotiamine with r2=0.999 and 0.998 respectively. LOD for both drugs were found to be 0.16 µg/ml and 0.3 µg/ml respectively. Method validation was done as per ICH guidelines. Keywords:Metformin Hydrochloride, Benfotiamine, Distilled water, UV Spectroscopy, Simultaneous equations, Q – Absorbance.

Authors and Affiliations

A. Tari, Padmavathi P. Prabhu, Paramita Das

Keywords

Related Articles

Immediate Massive Subcutaneous Emphysema: A Rare Complication of Percutaneous Dilatational Tracheostomy: A Case Report

Abstract: Percutaneous tracheostomy (PCT) has rapidly emerged in recent years; it is proven to be safe and less time-consuming when compared to surgical technique. In critical care settings, the use of PCT has been widel...

Protective Effects of Salacialehmbachii Aqueous Root Bark Extract Against Gastric Ulcer in Wistar Rats

Abstract: Salacialehmbachii is being used in traditional medicine for the treatment of gastric ulcer, this claim has not been scientifically substantiated or refuted. This work was therefore, designed to validate the ant...

Acute Pulmonary Embolism

Abstract: A 74 years old male patient presented with a two days history of dyspnea, orthopnea, pleuritic chest pain, hemoptysis, and fever after a history of fall from his bed with 2 fractured ribs two days ago. On physi...

HemophagocyticLymphohistiocytosis Associated Visceral Leishmaniasisin a Child: Evolution Favorable Under the Specific Treatment of Visceral Leishmaniasis

Abstract: Hemophagocyticlymphohistiocytosis (HLH) associated with visceral leishmaniasis (VL) in children is a rare clinicopathological entity, difficult to diagnose, fatal in the absence of treatment. It should be evoke...

A Detailed Anatomical Review on Mandibular Fracture

Abstract:Mandible is an important and the only movable bone in the craniofacial region. According to the old anatomists, it resembles a u-v shaped blunt bend. The mandible bears teeth, and this horseshoe-shaped/ v- bend...

Download PDF file
  • EP ID EP391769
  • DOI -
  • Views 72
  • Downloads 0

How To Cite

A. Tari, Padmavathi P. Prabhu, Paramita Das (2017). Analytical Method Development and Validation of Metformin Hydrochloride and Benfotiamine in Bulk and Marketed Formulations. Saudi Journal of Medical and Pharmaceutical Sciences, 3(12), 1322-1329. https://europub.co.uk/articles/-A-391769