ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF NADIFLOXACIN BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2014, Vol 6, Issue 9
Abstract
Objective: In the present work, a rapid, precise and sensitive HPLC Method with UV detection (237 nm) for analysis of Nadifloxacin in Bulk was developed.Methods: Chromatography was performed with a mobile phase containing a mixture of 0.05 %v/v trifluoro acetic acid and acetonitrile (65:35 v/v) with flow rate 1.2 ml min-l. The proposed method was validated as per the standard guidelines.Result: The retention time was found to be 12.3 min. In the range of 0.03-5 ppm, the linearity of Nadifloxacin shows a correlation co-efficient of 0.9997. Percentage recovery of the drug was found to be good (98-102%). Validation of the developed method was successful for precision, robustness, specificity and selectivity and ruggedness.Conclusion: The developed HPLC method was found to be simple, sensitive, precise, accurate and reproducible and can be successfully used for the quantitative estimation of Nadifloxacin in bulk.Â
Authors and Affiliations
Ayyagari Rama Murthy, Raghu Babu K. , Ngaji Ambabhai Vekariya
Keywords
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