Analytical Method Development for Dorzolamide Using UV Spectroscopy
Journal Title: Der Pharma Chemica - Year 2024, Vol 16, Issue 1
Abstract
The development and evaluation of an analytical technique for the UV spectroscopy based determination of Dorzolamide is the main focus of this study. Dorzolamide, a carbonic anhydrase inhibitor, is utilized to reduce intraocular pressure in the treatment of ocular hypertension and openangle glaucoma. The study involved establishing the maximum wavelength (λmax) of Dorzolamide HCL as 253 nm, confirmed by UV spectra in different media. The method's validation included linearity testing, where a calibration curve exhibited a strong correlation (R2=0.9995) in simulated tear fluid. Accuracy studies demonstrated a high precision of 98%-100%, while precision tests, both intra-day and inter-day, indicated a %RSD of less than 2%, confirming the method's reliability. The method's sensitivity was assessed with Limit of Detection (LOD) and Quantification (LOQ) found as 0.37 µg/ml and 1.12 µg/ml, respectively. Robustness and ruggedness assessments further supported the method's reliability, with %RSD values within acceptable ranges. The developed UV spectroscopy method for Dorzolamide analysis exhibits excellent accuracy, precision, sensitivity and robustness, making it a valuable tool for pharmaceutical quality control.
Authors and Affiliations
Sachin Kothawade1*, Shubhangi Albhar1 , Vijaykumar Wakale2 , Sandesh Bole1 , Kunal Raut3 , Prashant Patil1 , Jay prakash Suryawanshi 4 , Payal Navasupe 3 , Rachana Hajare 3 , Sharmila Kuskar1 , Vaibhav Wagh3 Vishal Pande
Keywords
Related Articles
- EP ID EP742471
- DOI 10.4172/0975-413X.16.1.172-177
- Views 15
- Downloads 0
Exploring the Potential Flavonoids as an Anti-Rheumatoid Arthritis Agent: An In silico Studies
Rheumatoid arthritis is an autoimmune disease which causes systemic complications, disability and even death. Its pathophysiology involves chronic inflammation of synovial membrane. New molecules in the biologic pathway...
Requalification of Friability Testing Apparatus
Re-qualification as a part of validation is the task performed to identify or check that utilities, equipment and ancillary systems can operate within limits for their intended use. Equipment qualification is a key eleme...
Determination and Quantification of an N-nitroso-Betaxolol in the Betaxolol Hydrochloride Active Pharmaceutical Ingredient by LC-MS/MS
A Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) method was developed for the quantification of N-nitroso-betaxolol in the betaxolol hydrochloride active pharmaceutical ingredient. Chromatographic separation w...
Development and Validation of Simplified RP-HPLC Method for Quantification of Candesartan Cilexetil in Commercial Formulations
In order to produce antihypertensive effects, Candesartan Cilexetil (CC), a candesartan inactive prodrug, was quickly converted into active candesartan after absorption in the Gastrointestinal (GI) tract. This research s...
Density, Ultrasonic Velocity, Viscosity and their Excess Parameters of Some Binary Liquid Mixtures of Cumene with Aromatic Hydrocarbons at 298.15 K
Density (ρ), viscosity (η) and sound velocity (u) of binary mixtures of ethyl benzene, toluene, mesitylene with cumene have been measured over the entire range of composition at temperature 298.15 K. From the experimenta...