Analytical method development for the simultaneous estimation of Hydrochlorothiazide and Atenolol in combined tablet formulation by RP-HPLC
Journal Title: Indian Journal of Research in Pharmacy and Biotechnology - Year 2017, Vol 5, Issue 5
Abstract
A Simple, accurate, rapid, sensitive and precise Rp-Hplc method has been developed for estimation of Hydrochlorothiazide and Atenolol in tablet dosage form using PDA detector. A Reverse phase Thermo Hypersil ODS c18 column (250mm × 4.6 mm I.D.,× 3µ particle size) with mobile phase consisting of Acetonitrile and Triethylamine buffer 100:900(v/v) [Ph 3±0.02 adjusted with orthophosphoric acid] was used. Retention time of Hydrochlorothiazide and Atenolol was found to be 1.9 and 5.8 minutes respectively. The linearity of the methods was found to be 50- 150µg/ml for Hydrochlorothiazide and 50-150µg/ml for Atenolol. The correlation co-efficient of Hydrochlorothiazide was found to be 0.9996 and the correlation co-efficient of Atenolol was found to be 0.9968.
Authors and Affiliations
K. Sridevi, A. Sirisha, M. Vinod Kumar, P. Parthiban
Keywords
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