Analytical method for development and validation of Dess-nitro and EDA in Entacapone tablet by using RP-HPLC method
Journal Title: Indian Journal of Research in Pharmacy and Biotechnology - Year 2017, Vol 5, Issue 5
Abstract
A simple, rapid, accurate and reproducible reverse phase high performance liquid chromatography method for the quantitative determination of Dess-nitro and EDA in Entacapone tablets is developed and validated.Entacapone (INN) is a medication commonly used in combination with other medications for the treatment of Parkinson's disease. Entacapone together with levodopa and carbidopa allows levodopa to have a longer effect in the brain and reduces Parkinson’s disease signs and symptoms for a greater length of time than levodopa and carbidopa therapy alone. Chromatography was carried out on a COSMOSIL πNAP column (250×4.6mm, 5µ) in gradient mode. The mobile phase consisted of buffer and methanol, pumped at a flow-rate of 0.8 ml/min. The UV detection was employed at 300nm. The retention time of dess-nitro and EDA was found to be 36.14 and 58.07 minutes respectively. The calibration curves were linear in the range. The method is accurate and precise with recovery of dess-nitro and EDA in the range of 95-105%. The proposed method which is rapid, simple and does not require any separation process has been successfully applied to the assay of commercial fixed dose formulations.
Authors and Affiliations
Nunsavathu Vasu Nayak, A. Sirisha, Periyasamy Parthiban, M. Vinod Kumar
Keywords
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- EP ID EP33484
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