ANTI-CANCER EFFECT OF OCIMUM SANCTUM ETHANOLIC EXTRACT IN NON-SMALL CELL LUNG CARCINOMA CELL LINE
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2016, Vol 8, Issue 4
Abstract
Objective: The present study was aimed to investigate the effects of alcoholic root extract of Ocimum sanctum, in human non-small cell lung carcinoma cell (NCI-H460).Methods: The effect of ethanolic extract of O. sanctum in NCI-H460 cell was investigated by the cell viability assay, generation of ROS in a cancer cell, apoptotic morphological changes and by mitochondrial membrane potential.Results: The cytotoxicity was observed by MMT assay. NCI-H460 cell was treated with various concentrations (10-150 µg/ml) of extract for 24 hr and 150 µg/ml showed a maximum decrease in cell viability. The extract (25-100µg/ml) showed significant increase ROS production in NCI-H460 cell. It greatly inhibits cell viability and colony forming capacity of NCI-H460 cell, possibly because of increased oxidative stress. An increased apoptotic cell in Ocimum sanctum further shows its anticancer nature. Loss of mitochondrial membrane potential is an early stage of apoptosis. Our results showed that extract treatment caused serve loss of in NCI-H460 cell.Conclusion: The present study suggests that O. sanctum extract act by increasing oxidative damage in NCI-H460 cells.Keywords: Ocimum sanctum, NCI-H460 lung carcinoma cells, MTT assay, Apoptosis, Oxidative damage
Authors and Affiliations
Sridevi M. , Bright John, Yamini K
Keywords
Related Articles
- EP ID EP577724
- DOI -
- Views 76
- Downloads 0
IN–VITRO ANTI-CANCER STUDY OF BIOTRANSFORMED MATERIAL (EXTRACT) OF CALENDULA OFFICINALIS BY MTT ASSAY
Objective: This work aims to determine the in-vitroanti-cancer activity of biotransformed material (extract) of calendula officinalisflowers.Methods: Various concentrations of the biotransformed material (extract) of cal...
ENHANCED TRANSDERMAL PERMEABILITY OF TELMISARTAN BY A NOVEL NANOEMULSION GEL
Objective: Telmisartan is an angiotensin II type I receptor blocker antihypertensive agent with 42% oral bioavailability. The aim of the present investigation was to develop a nanoemulsion gel to enhance bioavailability...
EVALUATION OF PHYTOCONSTITUENTS, IN VITRO ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF AGARICUS BISPORUS
Objective: The present study was aimed to investigate the phytochemicals, antimicrobial and antioxidant potential of wild edible mushroom (Agaricus bisporus). Methods: Initially, phytochemical screening was carried out t...
DIURETIC EFFECT OF THE ETHANOL EXTRACTS OF PHYLLANTHUS ACIDUS L (SKEELS) LEAVES IN WISTAR RATS
Objective: The objective of this study is to test diuretic effect of ethanol extract of P. acidus leaves in female Wistar rats using modification of Lipschitz method.Methods: The doses of the extract were 22.5, 45 and 90...
STABILITY STUDIES ON FLUCLOXACILLIN SODIUM IN CAPSULE DOSAGE FORMS
Objective: Flucloxacillin is easily broken down by moisture and this raises stability concerns of the drug in a country where humidity is very high.Stability studies on flucloxacillin sodium in capsule formulations were...