Anti–inflammatory, Antioxidant and Free Radical Scavenging Activities of Nyctanthes Arbor-Tristis Linn. Seed Extract Under In Vitro
Journal Title: Scholars Academic Journal of Biosciences - Year 2013, Vol 1, Issue 6
Abstract
Anti–inflammatory and Antioxidant properties of Nyctanthes arbor-tristis L. (Oleaceae) seeds extracted with Hexane, ethyl acetate and methanol revealed that ethyl acetate (Ny-El) extract showed potent effect than hexane and methanol extracts. The ethyl acetate extract exhibits 50% inhibition of anti–inflammatory activity at 7.19 ± 1.71 mg/mL. The total phenolic content of Ny-El extract was 206.81 ± 1.11 mgof catechol equivalents/g extract. Ny-El extract showed great scavenging activity on 2, 2-diphenyl-picrylhydrazyl (DPPH) (IC50 459.91±1.40 µg/mL), hydroxyl (IC50 363.32±1.58 µg/mL), nitric oxide (IC50 545.03 ± 1.69 µg/mL) andsuperoxide (IC50 338.82 ±1.72 µg/mL) radicals, as well as had high reducing power. Ny-El extract also showed a strong suppressive effect on lipid peroxidation. The metal chelating ability of Ny-El extract also showed strong inhibition effect when compared to the reference standard. Keywords: Anti–inflammatory, Nyctanthes arbor-tristis L, 2, 2-diphenyl-picrylhydrazyl, total phenolic content
Authors and Affiliations
S. Vajravijayan, A. Brabakaran, N. Thangaraju
Keywords
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