Anti-Inflammatory Effect of Xanthohumol in Combination With Praziquantel is Associated With Epithelial Mesenchymal Transition Factor Twist in Opisthorchiasis-Associated Cholangiocarcinoma
Journal Title: Biomedical Journal of Scientific & Technical Research (BJSTR) - Year 2019, Vol 12, Issue 4
Abstract
Cholangiocarcinoma (CCA) is the cancer of bile duct which is the common cancer and the major public health problem of northeastern region, Thailand. The major cause of CCA is chronic inflammation induced by liver fluke Opisthorchis viverrini (Ov) infection. Furthermore, one of key mechanism in the development of CCA is epithelial mesenchymal transition (EMT), which is characterized by loss of epithelial markers and gain of mesenchymal markers. One of the key EMT protein is Twist, an EMT transcription regulator, provides a significant prognostic marker that could be used as an EMT molecular target. Nowadays, there is no effective CCA treatment available. Several studies have attempted to use effective natural products for prevention and treatment of cancer including CCA. In this study aims to evaluate whether xanthohumol (XN) in combination with praziquantel, anthelmintic drug effect on inflammatory and Twist expression in cholangiocarcinogenesis in hamster model. Herein, four study groups of hamsters were conducted including;OV and NDMA treated (ON), b) ON with praziquantel receiving (ONP),; c) ON with xanthohumol receiving (XON) and; d) ON combination with praziquantel and xanthohumol receiving (XONP) at 60, 90, 120 and 180 days. The results revealed that inflammatory cells around bile ducts epithelial were decreased in ONP and XONP treated when compared with ON-treated group. However, decreasing of inflammatory cells was not seen in XON-treated hamsters. In addition, higher Twist expression was observed in ON group and the strongest staining of Twist was found at day 180. Interestingly, the reduction of Twist was seen in XON and ONP groups at day 90 when compared with the ON group. Moreover, our results exhibited an effective reduction of Twist expression around bile duct area for the XONP group. In summary, XN alone could not reduce inflammatory lesions around bile duct epithelial cells but in combination with PZQ can effectively decrease of inflammatory cell in liver. For EMT, XN and/or PZQ could potentially reduce Twist expression. Further studies are needed to confirm in depth the association between Twist and CCA prevention.
Authors and Affiliations
Phongsaran Kimawaha, Wassana Jamnongkan, Malinee Thanee, Watcharin Loilome, Anchalee Techasen
Keywords
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- EP ID EP587437
- DOI 10.26717/BJSTR.2019.12.002284
- Views 148
- Downloads 0
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