Antibacterial Activity of Flavonoids Extracted from Seeds of Pongamia pinnata Linn on Methicillin Resistant Staphylococcus aureus
Journal Title: Microbiology Research Journal International - Year 2015, Vol 10, Issue 1
Abstract
Aims: To assess the antibacterial property of seed crude extracts of Pongamia pinnata Linn and isolated flavonoids component from crude extract against Methicillin resistant Staphylococcus aureus obtained from clinical isolates. Study Design: Observational study. Place and Duration of the Study: Department of Allied health sciences, Department of Biochemistry and Department of Microbiology in Sri Devaraj Urs Academy Of Higher Education and Research ,Tamaka, Kolar, between February 2014 and march 2015. Methodology: Confirmed clinical isolates for MRSA were collected from Microbiology department to test the efficacy of crude extracts of seeds from Pongamia pinnata L. Methanolic crude extract has been preferably used for isolation of flavonoid content using Dimethyl Sulfoxide [DMSO] and methanol as ideal solvents during extraction process by column chromatography technique. Agar well diffusion method was performed to determine the antibacterial activity of crude seed extracts of Pongamia pinnata and isolated flavonoids by using quercitin as positive control for flavonoids. Vancomycin a glycopeptide powder used as gold standard for comparing bactericidal activity of quercitin, flavonoids and crude extracts of P. pinnata on MRSA. Results: The highest antibacterial activity (75-89%) was observed in crude extract of Pongamia pinnata in comparison to vancomycin considered as cent percent. Extracted flavonoids showed activity (66-92%) with respect to crude extract and (50-84%) with vancomycin and the activity (71-92%) with respect to quercitin when tested with concentration ranging from 25-400 µg/ml. Conclusion: This study showed that seed extracts of Pongamia Pinnata L and its phytochemical compound flavonoids showed potential antibacterial activity against MRSA using quercitin and vancomycin.
Authors and Affiliations
Mary Shobha Rani Inala, C. D. Dayanand, Nagarjuna Sivaraj, P. M. Beena, A. V. M. Kutty
Keywords
Related Articles
- EP ID EP354331
- DOI 10.9734/BMRJ/2015/20002
- Views 71
- Downloads 0
Differential Antimicrobial Effects of Conventional and Ethnobotanical Extracts from Vitellaria paradoxa Roots, Barks and Leaves
Aims: To determine the antimicrobial potentials of leaves, roots and barks of Vitellaria paradoxa “Shea-nut tree” used in traditional medicine for treatment of stomach ache and control of diarrhea. Place and Duration of...
Isolation, Characterization and Antibiotic Susceptibility of Mycoplasma hominis and Ureaplasma urealyticum from Infertile and Pregnant Women in Lagos, Nigeria
Background: Mycoplasma hominis and Ureaplasma urealyticum are potentially pathogenic agents, playing aetiologicroles in both genital infections and infertility. In human in-vitro fertilization systems, the presence of U....
Carrier Rate of Typhoid Organisms in Enugu, Nigeria
Aims: To determine the carrier rate of typhoid and paratyphoid organisms in Enugu community. Place and Duration of Study: The project was carried out in Enugu Urban in South Eastern part of Nigeria. Those enrolled in the...
In vitro Antibacterial Activity of Aqueous Extracts of Bidens pilosa L. (Asteraceae) from Nigeria
Aims: The aim of this work was to investigate the in vitro antibacterial activity of aqueous extracts of different organs of Bidens pilosa. Study Design: The design includes the extraction of crude extracts from the air-...
Antibiotics Susceptibility Profile of Extended Spectrum Beta Lactamase Producing Gram Negative Bacteria from Widal Positive Patients in Ekiti State University Teaching Hospital
Aims: To investigate the age occurrence of typhoid fever, antibiotics susceptibility profile of Extended Spectrum Beta-lactamase producing gram negative bacteria recovered from the blood culture of widal positive patient...