In vitro Antibacterial Activity of Aqueous Extracts of Bidens pilosa L. (Asteraceae) from Nigeria
Journal Title: Microbiology Research Journal International - Year 2015, Vol 8, Issue 4
Abstract
Aims: The aim of this work was to investigate the in vitro antibacterial activity of aqueous extracts of different organs of Bidens pilosa. Study Design: The design includes the extraction of crude extracts from the air-dried leaves, stems and roots samples of B. pilosa and the screening and determination of antibacterial activity of the extracts. Place and Duration of Study: The leaves, stems and roots of Bidens pilosa were exhaustively extracted separately with double distilled water. The extracts were tested for the presence of secondary metabolites. The antibacterial activity of extracts was tested against some clinical and environmental isolates using agar-disc diffusion and broth-microdilution methods. Results: The leaf extract exhibited significant inhibition on growth of the bacteria tested than the seed and root extracts. The mean zones of inhibition of the leaf extract ranged between (9.0±0.6 - 27.3±1.2) mm compared with (6.0±0.6- 11.7±0.6) mm and (6.0±0.6 - 16.3±0.6) mm for the seed and root extracts, respectively. The minimum bactericidal concentrations (MBC), 1.3 - >10 mg/ml was found to be equal or twice the minimum inhibitory concentrations (MIC), 0.6 - >10 mg/ml for the leaf extract and twice or greater for the stem and root extracts. Conclusion: The activity of the aqueous leaf extract of Bidens pilosa provides the scientific justification for the use of this plant in traditional medicinal.
Authors and Affiliations
Oladipupo A. Lawal, Kehinde O. Amisu, Segun K. Akinyemi, Adetayo A. Sanni, Mthokozisi B. C. Simelane, Rebamang A. Mosa, Andy R. Opoku
Keywords
Related Articles
- EP ID EP354553
- DOI 10.9734/BMRJ/2015/17900
- Views 97
- Downloads 0
Gram-Negative Bacteria from Pharynx and Nasal Cavity of Domestic Goats in Grenada, and Resistance of Mannheimia haemolytica and Bibersteinia trehalosi to Tulathromycin and Trimethoprim-Sulfamethoxazole
Aim: The present study was conducted to evaluate the occurrence of bacterial respiratory pathogens, particularly members of the family Pasteurellaceae in healthy domestic goats in Grenada, and to determine the antimicrob...
Unusual Oropharyngeal Asymptomatic Manifestations Caused by Atypical Pathogens Detected by PCR into Altered Ecosystems of an Infertile Couple
The young couple casually arrived at our observation presented two different and painless altered ecosystems. The female partner exhibited inflamed lingual surface, while the man manifested several genital ulceration are...
Effects of Ascorbic Acid and Levamisole on the Course of Newcastle Disease in Adult Japanese Quails
Aims: To investigate the effects of ascorbic acid, levamisole and their combination on the course of Newcastle disease in adult Japanese quails. Study Design: Randomized controlled trial. Methodology: The study was condu...
Differentiation in a Single-Tube PCR between Leishmania mexicana and Leishmania braziliensis in Clinical Samples
Aim: To assess the usefulness of a single-tube PCR end point for differentiating between Leishmania and Viannia subgenera in clinical samples of cutaneous leishmaniasis. Methodology: Impression smears of 65 patients from...
Landfill Waste Effluents Increase the Population and Diversity of Soil Microorganisms: The Case of Olusosun Landfill, Lagos, Nigeria
Aims: This study investigated the impact of landfill waste effluents on the population and diversity of soil microorganisms, and a comparative study between landfill soil and effluent-free field soil. Study Design: A co...