Hydrocarbons Degrading Potential of Stimulated Cultures of Bacteria Isolated from Humic Fresh Water Sediment of Eniong River in the Niger Delta of Nigeria

Journal Title: Microbiology Research Journal International - Year 2017, Vol 21, Issue 3


The Hydrocarbons degrading potential of stimulated cultures of bacteria isolated from humic fresh water sediment of Eniong River in the Niger Delta of Nigeria was studied. Preliminary screening of the humic hydrocarbonoclastic bacterial isolates revealed that among the 5 bacterial isolates (HSC1, HSC2, HSC3, HSC4 and HSC5), isolate HSC1 exhibited the strongest ability to utilize crude oil and was characterized to be Bacillus subtilis. In-vitro analysis of crude oil utilization of Bacillus subtilis- HSC1 when energized with various concentrations of sugar (glucose and sucrose) revealed variable levels of hydrocarbons utilization by monoculture of B. subtilis and enhanced degradation with biostimulation. The results of indirect assessment using the total viable cells revealed that the microbial biomass increased over time during degradation. The rate of increase was apparently higher in cultures stimulated with various concentrations of sugar (glucose and sucrose) than in the control and best growth were recorded on the 12th day when treated with 10% of glucose. Analysis of the optical density of the Bacillus subtilis during the degradation process revealed that the optical density increased with time. The pH of the test substrates decreased over time indicating a higher catabolic activity. The increase in acidity was higher in 15% and 20% glucose supplemented medium. The nutrient addition increased the bacterial cell numbers, optical density as well as the acidity (high decrease in the pH) of the test media between days 9 and 15 when compared with the rate derived from the test medium. In vitro degradation study carried out for the 15 days showed that, the degradation of crude oil and its component by B. subtilis was faster when stimulated with the different concentrations of sugar than when un-stimulated. The result showed a remarkable reduction in the total petroleum content of the test substrates treated with glucose and sucrose. The best results were obtained by treatment with 1 and 5% levels of the stimulants. At this level, the TPH content was reduced from 15.81 mg/kg observed for the control to 10.19 mg/kg (49.92% degradation) and 8.03 mg/kg (60.52% degradation) obtained from substrates stimulated with 5% glucose and 1% sucrose respectively. The high hydrocarbon degradation by stimulated culture of Bacillus subtilis- HSC1 implies that biostimulation can be harnessed for bioremediation purposes.

Authors and Affiliations

Umana, Senyene Idorenyin, Uko, Mfoniso Peter, Bassey, Maria Paul, Essien, Joseph Peter


Related Articles

Antimicrobial Potential of Dacryodes edulis against Selected Clinical Bacterial Isolates

The antimicrobial potential of Dacryodes edulis was investigated to determine the antimicrobial properties of the collected raw D. edulis pulp and seed extracts on some medically important human pathogens. The research w...

Detection of Heavy Metal Resistance Genes in an Environmental Pseudomonas aeruginosa Isolate

Aim: To detect heavy metals resistance genes in an environmental Pseudomonas aeruginoas (S 7) isolate. Place and Duration of Study: Department of Biology, Faculty of Applied Science, Umm Al-Qura University, Makkah...

Performance of Maize Storage Technologies in Benin: Fungal Ecology and Mycotoxin Contamination

Objectives: The present work was to study the fungal ecology of maize according to the various storage technologies and evaluate the ability of fungal isolates to produce mycotoxin. Materials and Methods: Seven maize st...

Biochemical Changes and Sensory Evaluation of Soy Iru Produced Using Starter Culture

Soybean (Glycine max L.) was processed into iru using Bacillus subtilis SBI 13 and Leuconostoc mesenteroides as starter culture and calabash as fermenting container. The fermenting soya beans were analysed for pH and tit...

Lactobacilli Cultures against Ochratoxin A Producing Moulds Isolated from Cocoa in the South West Region of Cameroon

The presence of ochratoxin A (OTA) in cocoa beans is a major health concern, due to its deleterious effects on humans and animals. During the traditional processing and storage of cocoa, fungi contamination occurs. Many...

Download PDF file
  • EP ID EP315910
  • DOI 10.9734/MRJI/2017/35647
  • Views 26
  • Downloads 0

How To Cite

Umana, Senyene Idorenyin, Uko, Mfoniso Peter, Bassey, Maria Paul, Essien, Joseph Peter (2017). Hydrocarbons Degrading Potential of Stimulated Cultures of Bacteria Isolated from Humic Fresh Water Sediment of Eniong River in the Niger Delta of Nigeria. Microbiology Research Journal International, 21(3), 1-13. https://europub.co.uk/articles/-A-315910