Hydrocarbons Degrading Potential of Stimulated Cultures of Bacteria Isolated from Humic Fresh Water Sediment of Eniong River in the Niger Delta of Nigeria
Journal Title: Microbiology Research Journal International - Year 2017, Vol 21, Issue 3
Abstract
The Hydrocarbons degrading potential of stimulated cultures of bacteria isolated from humic fresh water sediment of Eniong River in the Niger Delta of Nigeria was studied. Preliminary screening of the humic hydrocarbonoclastic bacterial isolates revealed that among the 5 bacterial isolates (HSC1, HSC2, HSC3, HSC4 and HSC5), isolate HSC1 exhibited the strongest ability to utilize crude oil and was characterized to be Bacillus subtilis. In-vitro analysis of crude oil utilization of Bacillus subtilis- HSC1 when energized with various concentrations of sugar (glucose and sucrose) revealed variable levels of hydrocarbons utilization by monoculture of B. subtilis and enhanced degradation with biostimulation. The results of indirect assessment using the total viable cells revealed that the microbial biomass increased over time during degradation. The rate of increase was apparently higher in cultures stimulated with various concentrations of sugar (glucose and sucrose) than in the control and best growth were recorded on the 12th day when treated with 10% of glucose. Analysis of the optical density of the Bacillus subtilis during the degradation process revealed that the optical density increased with time. The pH of the test substrates decreased over time indicating a higher catabolic activity. The increase in acidity was higher in 15% and 20% glucose supplemented medium. The nutrient addition increased the bacterial cell numbers, optical density as well as the acidity (high decrease in the pH) of the test media between days 9 and 15 when compared with the rate derived from the test medium. In vitro degradation study carried out for the 15 days showed that, the degradation of crude oil and its component by B. subtilis was faster when stimulated with the different concentrations of sugar than when un-stimulated. The result showed a remarkable reduction in the total petroleum content of the test substrates treated with glucose and sucrose. The best results were obtained by treatment with 1 and 5% levels of the stimulants. At this level, the TPH content was reduced from 15.81 mg/kg observed for the control to 10.19 mg/kg (49.92% degradation) and 8.03 mg/kg (60.52% degradation) obtained from substrates stimulated with 5% glucose and 1% sucrose respectively. The high hydrocarbon degradation by stimulated culture of Bacillus subtilis- HSC1 implies that biostimulation can be harnessed for bioremediation purposes.
Authors and Affiliations
Umana, Senyene Idorenyin, Uko, Mfoniso Peter, Bassey, Maria Paul, Essien, Joseph Peter
Molecular Biology Methods to Assess Microbiological Hazard Concerning Objects of Cultural Heritage
Microorganisms present in the environment pose a considerable hazard to historical objects and works of art. They are capable of settling on objects, and due to their ability to produce various enzymes, they can actively...
Identification of Ascitic Fluid Bacterial Pathogens in Spontaneous Bacterial Peritonitis in Nile Delta and Its Impact on Clinical Outcome of these Patients
Aims: This study aimed to identify ascetic fluid bacterial pathogens and their antibiotic resistance profile in Spontaneous Bacterial Peritonitis (SBP) patients in Nile delta and its impact on the clinical outcome of the...
Isolation and Characterization of Phosphate Solubilizing Bacteria from the Rhizosphere of Faba Bean (Vicia faba L.) in Meknes Region, Morocco
Aims: The objective of this research was to isolate phosphate solubilizing bacteria (PSB) from faba bean rhizosphere in Meknes region and evaluate in vitro their potential for P solubilization as well as their response t...
Biodeterioration Abilities of Microorganisms in Brake Fluids
Aims: To identify the parameters that get affected when brake fluids biodeteriorate and also to make known that brake fluids biodeteriorates into novel compounds. To also identify the organisms that deteriorates brake fl...
The Diagnosis of Salmonella typhi Co-infection from Blood Samples of Clinically Suspected Typhoid Fever Patients at Some Hospitals in Ondo State, Nigeria
Background: Blood samples of clinically suspected typhoid fever patients were screened for bacterial and fungal co-infection. Methodology: The study was conducted in three different hospitals in Ondo State, Nigeria viz;...