ANTIBACTERIAL ACTIVITY OF SELF-ETCH ADHESIVES

Apply

ANTIBACTERIAL ACTIVITY OF SELF-ETCH ADHESIVES

Journal Title: Journal of Evolution of Medical and Dental Sciences - Year 2017, Vol 6, Issue 87

Abstract

BACKGROUND Adhesion between tooth structure and the restoration is one of the most important factors determining the success of the restoration. The main reason for the failure and replacement of restorations is secondary caries. Dentin bonding agents with antibacterial effects may inhibit secondary caries formation and pulp inflammation by eliminating residual bacteria in and on dentin. Remineralisation by release of fluoride is representative, but the antibacterial effect is another important property because inactivation of bacteria means a direct strategy to eradicate the cause of dental caries. Aim- To evaluate and compare the antibacterial effect of two different Self-Etch Adhesives- FL-Bond II (fluoride releasing bonding agent), Single Bond Universal and 0.2% Chlorhexidine (Control) against Streptococcus salivarius and Streptococcus Mutans strains using agar well diffusion method. MATERIALS AND METHODS Two commercially available dentinal bonding agents to be selected, 0, 2% Chlorhexidine solution to be included as a positive control. The bacteria to be used in the experiments: Streptococcus mutans and Streptococcus salivarius isolated from Mutans Agar and Mitis Salivarius Agar and both strains to be grown overnight in brain heart infusion (BHI) broth. Strains were subcultured in Tryptone Soya Agar (Enriched Media). Samples were transferred to fresh BHI medium and incubated at 37°C for 6 to 8 hours to achieve an appropriate bacterial density. The susceptibility tests to be performed on blood agar and for the mutans streptococci, tests also to be conducted on mitis salivarius bacitracin (MSB) agar. For the agar well diffusion test, wells were cut on agar plates and were impregnated with 10 μL of each adhesive system and then immediately used. Plates were incubated at 37°C and examined at 24 hrs. The size of the inhibition zone (mm) was measured with sliding callipers. RESULTS The test results of the two different self-etch adhesives used were significantly different. CONCLUSION Both the tested adhesives showed promising antibacterial activity. In relation to S. mutans, FL Bond II showed similar antibacterial activity to Chlorhexidine. Although in relation to both strains (S. mutans and S. salivarius), SBU exhibited antibacterial activity; future studies comparing adhesives with and without MDP monomer have to be carried out to confirm its antibacterial activity.

Authors and Affiliations

Midhun Mohan G, Sulphi Abdul Basheer

Keywords

EP ID EP277863
DOI 10.14260/jemds/2017/1316
Views 100
Downloads 0