Antibacterial Activity of Vitex negundo L. against a Multidrug Resistant Pathogenic Bacterium
Journal Title: European Journal of Medicinal Plants - Year 2015, Vol 8, Issue 2
Abstract
Aims: The aim of this present study is to investigate antibacterial activity of extracts and/or essential oils of Vitex negundo L. leaves to an unknown pathogenic bacterium have been resistant to various groups of antibiotics Study Design: The study was design followed by previously studied and manuals of antimicrobial susceptibility test. Methodology: For antimicrobial susceptibility test disks diffusion method were used according to the guideline of European Committee on Antimicrobial Susceptibility Testing followed by McFarland standards. Various groups of antibiotics were used for conforming resistant of this unknown pathogen. The zone of inhibition and MIC was compared and measures with National Committee for Clinical Laboratory Standards (NCCLS) guidelines and reference data of previously studied. Results: According to NCCLS and data to our study revealed that UB201201 (unknown) was a multidrug resistant bacterium. Molecular identification discloses the bacterium were Bacillus cereus group. All extracts of Vitex negundo L. leaves (ethanol, chloroform, ethyl acetate and hexane) and essential oils were responsible for antibacterial susceptibility with inhibition zone of 29±0.7, 9±0.5, 8±0.3, 12±0.7, and 25±0.2 mm respectively. Conclusion: This study signifies ethanolic extract of Vitex negundo L. leaves as moderate antimicrobials and can be used for pharmaceutical and medicinal purpose. Therefore, isolation and identification of bioactive compounds from this plant will be an interest for human being.
Authors and Affiliations
Md. Mahfuz-Al- Mamun, Md. Monirul Islam, Rejoana Afrin Neera, Md. Rashed Nejum, G. M. Sala Uddin
In vitro Antioxidant, Antimicrobial, Insecticidal and Cytotoxic Activities of the Medicinal Plants: Allamanda cathartica and Mimusops elengi
A comparison of in vitro antioxidant, antimicrobial, insecticidal and cytotoxic activities of two medicinal plants, Allamanda cathartica and Mimusops elengi have been studied by spectrophotometric and disk diffusion meth...
A Survey on Medicinal Plant Usage by Folk Medicinal Practitioners in Different Villages at Nalitabari Upazilla, Sherpur District, Bangladesh
Medicinal plants are an important and often the only component in the formulations used by the folk medicinal practitioners of Bangladesh for treatment of various ailments. Folk medicinal practitioners, well known as Kav...
Effect of Aqueous Extract of Cassia alata Linn. on Oral Glucose Tolerance Test in Normal and STZ Induced Diabetic Mice
Aims: To check the effect of the aqueous extract of an antidiabetic plant Cassia alata Linn. on Oral Glucose Tolerance Test (OGTT) in glucose induced normal, and diabetic mice and to check if there is any toxic effect of...
Antibacterial Activity of Vitex negundo L. against a Multidrug Resistant Pathogenic Bacterium
Aims: The aim of this present study is to investigate antibacterial activity of extracts and/or essential oils of Vitex negundo L. leaves to an unknown pathogenic bacterium have been resistant to various groups of antibi...
In vitro Anti-dermatophyte Activities of Crude Extracts of Cassia alata
Aim: To study the in vitro anti dermatophyte activities of methanol and cold water extracts of Cassia alata. Study Design: Experimental. Place and Duration of Study: Department of Applied Microbiology and Brewing, Nnamd...