Effect of Aqueous Extract of Cassia alata Linn. on Oral Glucose Tolerance Test in Normal and STZ Induced Diabetic Mice
Journal Title: European Journal of Medicinal Plants - Year 2016, Vol 15, Issue 1
Abstract
Aims: To check the effect of the aqueous extract of an antidiabetic plant Cassia alata Linn. on Oral Glucose Tolerance Test (OGTT) in glucose induced normal, and diabetic mice and to check if there is any toxic effect of the extract. Study Design: OGTT was carried out in two main groups (normal and diabetic) of mice, with 3 sub-groups. The effect of CAAE (Cassia alata Aqueous Extract) on OGTT in normal mice group was assessed at different time intervals (0 min, 30 min, 60 min, 90 min and 120 min). Oral acute toxicity test of the extract was performed in three groups of mice. Place and Duration of the Study: The study was conducted at Assam University, Department of Biotechnology, Assam, India, between February and August, 2014. Methods: OGTT was performed following the method of Badole 2006, in STZ induced diabetic mice group and normal mice group. Acute oral toxicity test was performed based on OECD guidelines, with reference to behavioural aspects, in Swiss Albino mice. Results: CAAE did not produce any mortality and sign of lethality throughout the study period of 14 days. In normal mice the percentage change in BGL ranges from 91.42% - 40.85% in NC (Normal Control) mice; 84.85% - 21.90% in GTNM Glibenclamide treated Normal Mice); and 88.66% - 43.28% in AqETNM (Aqueous Extract treated Normal Mice). In Diabetic group the percentage change in BGL ranges from 66.51% - 46.38% in DC (Diabetic Control) mice; 53.51% - 12.50% in GTDM (glibenclamide treated Diabetic Mice); and 55.21% - 0.33 in AqETDM (Aqueous Extract treated diabetic Mice). Conclusion: The toxicity test result indicates that the extract is nontoxic and can be used in further bioactivity test. The study reveals the positive effect of extracts in maintaining glucose homeostasis in mice.
Authors and Affiliations
Priyadarshini Laishram, Mazumder Pranab Behari, Pushparani Heisanam, Manabendra Dutta Choudhury
Keywords
Related Articles
- EP ID EP349650
- DOI 10.9734/EJMP/2016/24704
- Views 109
- Downloads 0
Alternative to Conventional Diabetic Management: The Antihyperglycaemic potential of an Ethyl Acetate Fraction Extract of Holarrhena floribunda
Aims: Antihyperglycaemic effects of an ethyl acetate fraction of the leaf extract of Holarrhena floribunda on normal and streptozotocin-induced diabetic rats. Study Design: Healthy adult albino Wistar rats (weighing 100-...
Microbiological Contamination and Anti-bacterial Traits of Common Oral Herbal Medicinal Products within Dhaka Metropolis
Aims: Present study endeavored to examine the growth and survival of microorganisms within 6 categories of oral herbal medicines commonly used by the community within Dhaka metropolis. Methodology: Samples were analyzed...
In vitro Shoot Multiplication of Medicinally Important Plant Andrographis lineata Nees via Nodal Explants
Andrographis lineata Nees (Acanthaceae) is an endemic herb rich in flavonoids and diterpenoids which have pharmaceutical importance. Seedling explants source has not been studied for micropropagation and hence the presen...
The Effect of Different Drying Methods on the Phytochemicals and Radical Scavenging Activity of Ceylon Cinnamon (Cinnamomum zeylanicum) Plant Parts
Aim: The research aimed at ascertaining whether the different drying methods and plant parts have effect on the radical scavenging activity and phytochemical properties of cinnamon as an herb/spice. Methodology: Fresh s...
Fertilizer Source in Biomass Production and Quality of Essential Oils of Thyme (Thymus vulgaris L.)
Aims: The goal of this study was performed in order to test the effect of the source of crop fertilization and harvest days in the production of biomass and measure the qualitative and quantitative properties of the prin...