In vitro Shoot Multiplication of Medicinally Important Plant Andrographis lineata Nees via Nodal Explants
Journal Title: European Journal of Medicinal Plants - Year 2016, Vol 17, Issue 3
Abstract
Andrographis lineata Nees (Acanthaceae) is an endemic herb rich in flavonoids and diterpenoids which have pharmaceutical importance. Seedling explants source has not been studied for micropropagation and hence the present investigation has been taken up. An efficient protocol was developed for aseptic seed germination and in vitro shoot multiplication from different aseptic seedling explants. 30 days old aseptic seedlings grown on half strength MS medium were used as source of explants. Shoot tips, nodal segment, cotyledonary nodes, leaves, internodes and root segments were cultured on MS medium supplemented with BA 8.87 µM for testing for their responsivity. Nodal segments gave a maximum response when compared to other seedling explants and therefore were used in further experiments. Nodal segments were cultured on MS medium supplemented with various concentrations of cytokinins individually and in combination with auxins. Maximum number of 32.04 shoots per nodal explant were produced after 30 days of culturing on BA 8.87 µM + Indole-3-Butyric acid (IBA) 6.54 µM. Number of shoots further increased by sub culturing into fresh medium containing MS medium supplemented with BA 8.87 µM. Shoots were rooted on half strength MS medium and rooted plants were hardened in polycups containing sterile peat moss, farmyard manure and soil (1:1:1) with 75% survival rate.
Authors and Affiliations
V. Aruna, T. Pullaiah
Keywords
Related Articles
- EP ID EP349541
- DOI 10.9734/EJMP/2016/30411
- Views 116
- Downloads 0
Total Antioxidants Status, Selenium Concentration, Catalase and Glutathione Peroxidase Activities in Rabbits Fed a High-Garlic Diet
Aim: Results from previous studies on the effects of intake of garlic on the body antioxidant system appear inconclusive. This study measured glutathione peroxidase (GPx) and catalase (CAT) activities, Total Antioxidant...
Effect of Natural Cocoa Powder Supplementation on Oxidative Stress in Healthy Ghanaians
Introduction: Natural cocoa powder (NCP) has been recognized to possess significant amounts of flavonoids and methylxanthines with healthful benefits. Little or no work has been done on adult Ghanaians involving suppleme...
In vitro Modulation of Pancreatic Insulin Secretion and Extra Pancreatic Insulin Action, Enzymatic Starch Digestion and Protein Glycation by Terminalia chebula Extracts
Aim: Traditional plant treatments have been used throughout the world for the therapy of diabetes mellitus. Study Design: Using multiple in vitro models; this study was designed to investigate the efficacy and mode of a...
Pharmacognostic and HPTLC Fingerprint Profile of the Root of Aristolochia indica Linn. and Quantification of the Marker Compound
Introduction: Root of Aristolochia indica Linn. has long been used as an oxytoxic agent to aid women in child birth and as abortifacient in Indian folk medicine. It is also one of the ingredients in some traditional Ayur...
Acaricidal Activities of Hyptis suaveolens and Ocimum sanctum Against African Dog Tick (Rhipicephalus sanguinneus)
Aim: To determine the acaricidal activities of some solvent extracts (chloroform, methanol and n-hexane) and crude extracts of Hyptis suaveolens and Ocimum sanctum against African dog tick (Rhipicephalus sanguinneus). S...