Antibacterial and Antifungal Efficacy of Partially Partitioned Fractions of Spondias mombin (Linn) Extracts (Root, Leaf and Stem Bark) against Clinical and Environmental Isolates
Journal Title: UNKNOWN - Year 2019, Vol 8, Issue 1
Abstract
The purpose this research work is to determine the antibacterial and antifungal assay of partitioned fractions of S. mombin (Linn) extracts against clinical and environmental isolates. The root, leaf and stem-bark of S. mombinwere harvested and air-dried. The dried S. mombin was milled into powdered form using manual grinder. Powdered S. mombin (1 kg) each of the different S. mombin parts was extracted with 3 L of 70% (v/v) ethanol, ethyl acetate and distilled water for 72 h at room temperature. The SMRE and SMREA were used to code for root part; SMLE and SMLEA for the leaf part; and SMSBE and SMSBEA for the stem-bark part, each was fractionated on column chromatography with silica as the stationary phase using n-hexane, ethyl acetate and ethanol as the eluting solvent to obtain n-hexane, ethyl acetate and ethanol fractions. Antimicrobial and antifungal screened was observed using agar well diffusion test. The result obtained showed that in partially purified ethyl acetate leaf extracts, Fraction (F1SMLEAH) showed significant inhibitory effect (p ≤ 0.05) on all the test bacteria, except Klebsiella pneumoniae and Salmonella typhi at concentrations of 20.0-2.5 mg/ml, Fraction (F2SMLEAEA) was not effective against Salmonella cholleraesuis, B. substilis, Citrobacter koseri and Salmonella typhi. Fraction(F3 SMLEAE) showed little or no inhibitory effect on most of the bacteria at all the concentration used. It can be deduced that in partially purified ethanolic leaf extracts, Fraction (F1 SMLEH) showed inhibitory effect on Burkholderia cepacia. All the organisms were not susceptible to all the fractions except F1 which had diameter of zones of growth inhibition ranging between 4.0-1.0 mm at 5 mg/ml-0.625 mg/ml on Mycobacterium abscessus.Partially purified ethanolic stem bark extracts, antifungal activity of the partially purified ethanolic extracts of S. mombin, Fractions (F1 SMLEH, F2 SMLEEA) and F3 SMLEE), significant antifungal activity (p ≤ 0.05) was observed at 20.0 mg/ml with most test fungi. Trichoderma horizionum was not susceptible to all the three fractions, while Aspergillus niger and Syncephala strumracemosumwere susceptible to only fraction (F2 SMLEEA). Fractions (F1 SMSBEH, F2 SMSBEEA), F3 SMSBEE) on the test as the eluting solvent. Significant inhibitory effect (p ≤ 0.05) was observed in all the fractions at 20 mg/ml against most of the test bacteria. While, zones of growth inhibition of the various fractions varied with the test bacteria with the highest diameter zone of 8.0 mm recorded in fraction F1 against Salmonella typhi. Fractions (F2 SMSBEEA and F3 SMSBEE) possessed significant inhibitory effect (p ≤ 0.05) at 20.0-5.0 mg/ml on the test fungi, except Candida kruise and Rhizopus stonifer. The plant part by solvent interactive effect was significant (p<0.05), suggesting that the MICs and MBCs of test bacteria were observed at 0.3125 and 0.1562 mg/ml and MICs and MFCs test fungal were observed at 0.3125 and 0.1562 mg/mlrespectively, The various plants differ significantly according to extraction solvent, These findings demonstrate the possible effectiveness of the S. mombin plant, especially its stem bark extracts, in treating microbial infections.
Authors and Affiliations
Oludare Temitope Osuntokun, Idowu TO, Gamberini Maria Cristina
Keywords
Related Articles
- EP ID EP672248
- DOI -
- Views 34
- Downloads 0
Stem Cell in Lingual Epithelia and Papillae
In mammalian tongue, all the compartments of the lingual epithelium are turned over throughout adult life. There is a continual requirement for proliferation to replace cells lost by differentiation within each compartme...
Molecular Hybridization - An Emanating Tool in Drug Design
Most of the diseases are multifactorial in nature. Moreover, the exploration of new drugs with appropriate absorption, distribution, metabolism, and excretion along with effective pharmacological activity and less toxici...
In Vitro Antibacterial Activity and Phytochemical Analysis of Zingiber officinale L. Rhizome Extracts
The aim of this research was to determine antibacterial activity and phyto-chemical screening of ethanolic extracts of Zingiber officinale. Fresh rhizomes of Zingiber officinale (Ginger) were collected from Kachia Local...
Potential Anticancer Agents: Design, Synthesis of New Pyrido[1,2-a]benzimidazoles and Related Derivatives Linked to Alkylating Fragments
The incentive of the present work has been primarily directed towards the design and synthesis of some novel pyrido[1,2-a]benzimidazoles with specific functionalities believed to have alkylation ability. This combination...
Synthesis and Antioxidant Activity of Mixed Ligand Complex of Quercetin and Aspartic Acid with Cobalt(II)
Mixed ligand complexes are widely used in the field of pharmaceutical science due to their curing nature to various diseases. The present study was aimed to synthesize and explore the antioxi...