Antibacterial and cytotoxic activity screening of leaf extracts of Vitex<br /> negundo (Fam: Verbenaceae)
Journal Title: Journal of Pharmaceutical Sciences and Research - Year 2009, Vol 1, Issue 4
Abstract
The work described in this paper details the biological investigation on Vitex negundo, species of Verbenaceae. The methanol crude extract of Vitex negundo was fractionated with kupchan method and pet-ether and carbon tetrachloride were made for screening the antimicrobial and antitumor potentials using disc diffusion method and brine shrimp lethality bioassay respectively. An established antibiotic (Kanamycin, 30μg/disc) and cytotoxic agent (Vincristine sulphate) were used to compare the results. From the graphs the LC50 (50% mortality) values were found as 12.5g/ml, 1.55g/ml and 1.56g/ml for methanolic crude extract, pet-ether and carbon tetrachloride fractions respectively. LC90 was also determined from the graph to establish the therapeutic index and the value were found 150.0g/ml, 50g/ml and 50g/ml for methanolic crude extract, pet-ether and carbon tetrachloride fractions respectively. The four fractions were also assayed for antimicrobial screening and all the fractions showed most prominent zone of inhibition against a number of bacterial and fungal strains. Especially in comparison to the standard kanamycin, all fractions gave prominent zone of inhibition against Bacillus subtilis, Bacillus megaterium, Salmonella typhi, Vibrio mimicus and a fungal strain, Aspergillus niger.
Authors and Affiliations
Chowdhury J. A. , Islam S. I. , Asifuzzaman Sk. , Islam M. K.
Keywords
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