Antibacterial Efficacy of the In-Use Dilutions of Common Disinfectants against Pseudomonas aeruginosa Isolates in a Tertiary Care Hospital in Calabar, Nigeria
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2018, Vol 13, Issue 3
Abstract
Background: Microorganisms are readily transmitted through various means including contaminated materials or instruments used in patient care. Disinfection is an important component of infection control. Use of chemical disinfectant has led to drastic reduction in transmission and spread of hospital pathogens. Concentration has a great influence on the potency of disinfectants. An evaluation study to determine the effectiveness of in-use dilutions of common disinfectants used in the University of Calabar Teaching Hospital on Pseudomonas aeruginosa strains was carried out. Method: P.aeruginosa isolates were obtained from inanimate sources in the hospital and characterized with a Gram Negative-ID system using P.aeruginosa ATCC 27853 as control. In-use dilutions of four disinfectants, Dettol, izal, savlon and bleach, were obtained from house-keeping staff in the hospital wards. The Rideal-walker phenol coefficient test was used to determine disinfectant efficacy while thequantitative suspension test was used for their antimicrobial evaluation. Results: Izal had the highest phenol coefficient of 3.25 savlon, bleach and Dettol had phenol coefficient of 2.75, 0.75 and 0.50 respectively. Savlon eliminated all viable cells of P.aeruginosa after five minutes exposure.Izal eliminated all cells after 20 minutes while Dettol and bleach had similar activities and eliminated cells after 15 minutes exposure. Conclusion: The in-use dilution of izal was ineffective although it had the highest phenol coefficient of the four disinfectants tested. The concentrations of in-use solutions of the three disinfectants should be reviewed to enhance their usefulness in infection prevention and control in the hospital.
Authors and Affiliations
Aniekan-Augusta O. Eyo, Emmanuel O. Ibeneme, Ofonime M. Ogba, Anne E. Asuquo
Keywords
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- EP ID EP395155
- DOI 10.9790/3008-1303028891
- Views 84
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