Antibiogram and heavy metal tolerance of bullfrog bacteria in Malaysia

Journal Title: Open Veterinary Journal - Year 2011, Vol 1, Issue 10


Bacterial isolates from 30 farmed bullfrogs (Lithobates catesbeianus) weighing 500-600 g at Johore, Malaysia with external clinical signs of ulcer, red leg and torticollis were tested for their antibiograms and heavy metal tolerance patterns. A total of 17 bacterial species with 77 strains were successfully isolated and assigned to 21 antibiotics and 4 types of heavy metal (Hg2+, Cr6+, Cd2+, Cu2+). Results revealed that bacteria were resistant against lincomycin (92%), oleandomycin (72.7%) and furazolidone (71.4%) while being susceptible to chloramphenicol and florfenicol at 97.4%. The multiple antibiotic resistance (MAR) index for C. freundii, E. coli and M. morganii was high with the value up to 0.71. Bacterial strains were found to exhibit 100 % resistance to chromium and mercury. High correlation of resistance against both antibiotics and heavy metals was found (71.4 to 100%) between bullfrog bacteria isolates, except bacteria that were resistant to kanamycin showed only 25% resistance against Cu2+. Based on the results in this study, bacterial pathogens of bullfrog culture in Johore, Malaysia, were highly resistant to both antibiotics and heavy metals.

Authors and Affiliations

L. W. Tee| Faculty of Agrotechnology and Food Science, Universiti Malaysia Terengganu, 21030 Terengganu, Malaysia, M. Najiah| Faculty of Agrotechnology and Food Science, Universiti Malaysia Terengganu, 21030 Terengganu, Malaysia


Related Articles

Splenophrenic portosystemic shunt in dogs with and without portal hypertension: can acquired and congenital porto-caval connections coexist?

The possible existence of the same pattern of porto-caval connection in dogs having a single congenital portosystemic shunt (CPSS) and in dogs having multiple acquired portosystemic shunt (MAPSS) secondary to portal hyp...

Prenatal transmission of scrapie in sheep and goats: A case study for veterinary public health

Unsettled knowledge as to whether scrapie transmits prenatally in sheep and goats and transmits by semen and preimplantation embryos has a potential to compromise measures for controlling, preventing and eliminating the...

Cadmium affects the mitochondrial viability and the acid soluble thiols concentration in liver, kidney, heart and gills of Ancistrus brevifilis (Eigenmann, 1920)

The freshwater fish Ancistrus brevifilis, which is found in Venezuelan rivers, is considered a potential sentinel fish in ecotoxicological studies. The cadmium (Cd) effect on the mitochondrial viability (MV) and acid so...

Search for the genome of bovine herpesvirus types 1, 4 and 5 in bovine semen

Bovine herpesvirus type 1 (BoHV-1) causes respiratory and reproductive disorders in cattle. Recently, bovine herpesvirus type 5 (BoHV-5) and bovine herpesvirus type 4 (BoHV-4) have been identified to be associated with...

Innocuity and immune response to Brucella melitensis Rev.1 vaccine in camels (Camelus dromedarius)

A field trial was conducted in a camel brucellosis-free herd to evaluate antibody response to the Brucella melitensis Rev.1 vaccine in camels and assess shedding of the vaccine strain in milk. Twenty eight camels were d...

Download PDF file
  • EP ID EP9249
  • DOI -
  • Views 439
  • Downloads 43

How To Cite

L. W. Tee, M. Najiah (2011). Antibiogram and heavy metal tolerance of bullfrog bacteria in Malaysia. Open Veterinary Journal, 1(10), 39-45.