ANTIBIOTIC RESISTANCE AND BIOFILM FORMATION AMONG NOSOCOMIAL PATHOGENS IN A TERTIARY CARE HOSPITAL
Journal Title: Journal of Evolution of Medical and Dental Sciences - Year 2017, Vol 6, Issue 84
Abstract
BACKGROUND Biofilm formation is an important mode of growth because it is associated with the chronic nature of the subsequent infections, and it helps in inherent resistance to antibiotic chemotherapy. Various nosocomial infections which are associated with the indwelling devices are the source as their surfaces become a niche for biofilm producing microorganisms. MATERIALS AND METHODS Various tip cultures received in microbiology lab from OPD and IPD patients were processed for isolation, identification and antimicrobial sensitivity. Different tip cultures which were included in the present study were urinary catheter tip, tracheostomy tip, endotracheal tip, chest tube tip and central line tip. After identification, the different isolates were investigated for antibiotic susceptibility testing by Kirby-Bauer disc diffusion method on Mueller-Hinton Agar (MHA) and the zones were interpreted as per CLSI guidelines (4, 5). Multi-drug Resistant (MDR) organisms viz. S. aureus, K. pneumoniae, E. coli, P. aeruginosa and A. baumannii were then further tested for biofilm production by microtiter plate method and tube method. RESULTS Highest percentage of biofilm producing organisms was of K. pneumoniae, followed by A. baumannii and P. aeruginosa whereas least strains were of S. aureus. Tube adherence method detected 2.33% strong producers whereas microtiter plate method detected 3.33% strong producers, 45% moderate biofilm producers were produced by tube adherence method and 58.33% moderate biofilm producers were produced by microtiter plate method. 52.67% and 38.33% non-biofilm producers were produced by tube adherence and microtiter plate method respectively. The total biofilm producing percentage came out to be 62%. CONCLUSION Nosocomial (hospital-acquired) infections continue to be a burden on the economy and on the life expectancy of patients. The increasing evidence on ability of nosocomial pathogens to form biofilms on various indwelling devices makes it imperative for the clinicians for early detection of biofilms and setting up of antibiotic policy to treat these pathogens in the hospital settings.
Authors and Affiliations
Kamaldeep Kaur, Amarjit Kaur Gill, Prabhjot Kaur Gill, Avneet Kaur Heyar
Keywords
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- EP ID EP276388
- DOI 10.14260/jemds/2017/1268
- Views 58
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