Anticancer activity test of ethyl acetate extract of endophytic fungi isolated from soursop leaf (Annona muricata L.)
Journal Title: Asian Pacific Journal of Tropical Medicine - Year 2017, Vol 10, Issue 6
Abstract
Objective: To analyze anticancer activity of an ethyl acetate extract of endophytic fungi isolated from soursop leaf (Annona muricata L.). Methods: Anticancer activity of fungal extracts was determined by observing its toxicity against MCF-7 (Michigan Cancer Foundation-7) cells in vitro by using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay method. At an extract concentration of 100 mg/mL, 4 isolates out of 12 showed high activity against the cancer cell growth. The four isolates were then selected for further IC50 determination, by measuring the inhibition of cancer cell proliferation at extract concentration of 25 mg/mL, 50 mg/mL, 100 mg/mL, 200 mg/mL and 400 mg/mL. Results: Results showed that isolate Sir-G5 had the highest anticancer activity with an IC50 of 19.20 mg/mL. The best isolates were screened again using a normal cell (Chang cells) to determine its toxicity against normal cells. Results indicated that the extracts do not affect the proliferation of normal cells. Molecular identification showed that the fungal isolate Sir-G5 has a close relationship with Phomopsis sp. Conclusions: The endophytic fungi isolated from soursop leaf has the potential to be used as a source of anticancer agents.
Authors and Affiliations
I Made Artika
Keywords
Related Articles
- EP ID EP267433
- DOI 10.1016/j.apjtm.2017.06.004
- Views 100
- Downloads 0
Effect of Egyptian propolis on cryptosporidiosis in immunosuppressed rats with special emphasis on oocysts shedding, leukogram, protein profile and ileum histopathology
Objectives: To investigate the activity of Egyptian propolis extracts (ethanol and water) on cryptosporidiosis in experimentally infected dexamethasone-immunosuppressed rats. Methods: A total of 180 male rats (190–220) g...
Targeted inhibition of Notch1 gene enhances the killing effects of paclitaxel on triple negative breast cancer cells
Objective: To study the influence of targeted inhibition of Notch1 gene on the killing effects of paclitaxel on triple negative breast cancer cells. Methods: The triple negative [estrogen receptor (ER)/progesterone recep...
Phyllanthus acidus (L.) Skeels and Rhinacanthus nasutus (L.) Kurz leaf extracts suppress melanogenesis in normal human epidermal melanocytes and reconstitutive skin culture
Objective: To determine the effect of extracts from Phyllanthus acidus (P. acidus) (L.) Skeels and Rhinacanthus nasutus (R. nasutus) (L.) Kurz leaves on melanogenesis and the underlying mechanism in normal human epiderma...
Protoscolicidal and immunomodulatory activity of Ziziphora tenuior extract and its fractions
Objective: To evaluate the scolicidal and immunomodulatory effect of the Ziziphora tenuior (Z. tenuior) extract and its fractions. Methods: Protoscolices were treated with six concentrations (3, 5, 10, 25, 50, and 100 mg...
Potentiating activity of rhein in targeting of resistance genes in methicillin-resistant Staphylococcus aureus
Objective: To investigate the synergistic effect between rhein (RHE) and oxacillin against Staphylococcus aureus (MRSA) at the gene level. Method: A minimum inhibitory concentration and checkerboard dilution test were co...