Antiendotoxin Immunity and C-Reactive Protein Level in Patients with Diffuse Toxic Goiter and Heart Pathology
Journal Title: Міжнародний ендокринологічний журнал - Year 2014, Vol 2, Issue 58
Abstract
The changes in cardiovascular system take one of the leading places in the clinic of diffuse toxic goiter (DTG) and can define prognosis for a disease. Endotoxin (ET) can be the inductor of a systemic inflammation in patients with DTG and aggravate its clinical course. From there, the purpose of this work was to study humoral and cellular antiendotoxin immunity and C-reactive protein (CRP) level in patients with heart pathology. The level of antiendotoxin antibodies and CRP were studied by enzyme-linked immunosorbent assay. As an antigen, we used ET of the Gram-negative Escheriсhia coli K30 (09:K30:P12), isolated from bacterial biomass by method of water phenol extraction and additionally purified from RNA admixtures using cetavlon processing (Serva, Германия). Receptors to ET were determined by flow lase cytometry using a two-color immunofluorescence analysis using a monoclonal anti-CD14-PE IOTest® (CD14) and conjugate of lipopolysaccharide Escherichia coli K235 with fluorescein isothiocyanate (ET-P). For the study we allocated 3 groups of patients. The first group included 11 patients with DTG who do not have heart disease, the second group consisted of 47 patients with DTG and endocrine cardiomyopathy complicated by arrhythmia, and the third group — 13 patients with DTG and concomitant ischemic heart disease (IHD). Control group consisted of 33 apparently healthy subjects. It is found that in all patients with DTG and heart pathology, a possible reduction of anti-ET-sIgA, anti-ET-IgM and anti-ET-IG is observed in comparison with the control group. This is accompanied by an increase in CRP levels in these groups, especially in patients with DTG and IHD, in whom its level was significantly higher than in patients with DTG and without heart disease. Analysis of literature data and our results confirm the hypothesis that extravasation of the excess amount of ET into the bloodstream depletes reserves of synthesis of specific antibodies, assists enhance of the systemic inflammatory response and the emergence of typical cardiac pathology in examined patients, which makes the course of the underlying disease difficult.
Authors and Affiliations
V. A. Beloglazov, Yu. Yu. Kulagina
Keywords
Related Articles
- EP ID EP226814
- DOI -
- Views 41
- Downloads 0
Primary hyperaldosteronism. Mechanisms and consequences of pathological influence on an organism (review of literature)
The work is based on the analysis of world literature data on primary hyperaldosteronism. The data on mechanisms of pathological influences of excessive autonomous aldosterone secretion on the body systems are given. The...
Immune and anti-inflammatory factors in the mechanism of therapeutic effect of metformin in type 2 diabetes mellitus (analytical review including the results of own researches)
The review deals with the analysis of modern literature, including the results of own researches on the mechanism of the therapeutic effect of metformin in patients with type 2 diabetes mellitus (DM2) and its complicat...
Clinical and laboratory criteria for type 2 diabetes mellitus in children
The purpose of this review was the analysis of literature data on clinical and laboratory criteria for type 2 diabetes mellitus in children. A review of scientific literature was conducted using Pubmed as the search engi...
Influence of diffuse nontoxic goiter on placental insufficiency, course of gestation and childbirth
Background. There are significant disorders in the functioning of the mother-placenta-fetus system in women with thyroid pathology, especially in chronic iodine deficiency. This leads to complications of pregnancy, child...
Evaluating the quality of life in children and adolescents with type 1 diabetes mellitus on pump insulin therapy, considering the carbohydrate value in Uzbek national foods
Background. Type 1 diabetes mellitus (DM) in children and adolescents is a complex problem throughout the world. The onset of the disease at an early age and development of acute and chronic complications already at youn...