ANTIFUNGAL ACTIVITY OF FIVE STRAINS OF SOIL MICROORGANISMS ISOLATED FROM TANGKUBAN PERAHU MOUNTAIN
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2015, Vol 7, Issue 2
Abstract
Objective: The objective of this research was production of antifungal by microorganism isolated from soil of Tangkuban Perahu mountain.Methods: The microorganisms were isolated from soil by the serial dilution method and identified by observing morphological, biochemical and molecular characteristics.Production of antifungal metabolites was done by fermentation. Screening for bioactivity of isolates was done by testing the fermentation broth against Microsforum gypseum, the metabolite was extracted with ethyl acetate. Antifungal activities from the ethyl acetate extract were tested by agar diffusion and microdillution methods.Results: Five strains of microorganisms were isolated from soil taken from Tangkuban Perahu mountain and were investigated in this study, designated TP1, TP2, TP3, TP4, and TP5, respectively. These isolates were shown to be closely related to Nocardia sp. YIM 65630 GU367167.1 (90%), Streptomyces galbus NR 026178.1 (99%), Aspergillus unguis FJ878626 (86%), Paecilomyces marquandii FJ765026 (100%), and Nocardia niigatensis NR 043916.1 (95%). The result of the bioactive screening showed that all isolates had antifungal activities, with the range of diameter of inhibition of 7-18 mm against M. gypseum, and TP2 has diameter of inhibition of 18 ± 0.05 mm.Conclusion: The highest antifungal activity showed by TP2 strain. The ethyl acetate extract of TP2 strain against Microsporum gypseum has the MIC of 4.7 µg/ml and diameter of inhibition was 36 ± 0.10 mm.Â
Authors and Affiliations
Ukan Sukandar, Desak Gede Sri Andayani, Elin Yulinah Sukandar, I Ketut Adnyana
Keywords
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