Antifungal Susceptibility Pattern among Candida species: An Evaluation of Disc Diffusion and Broth Micro-dilution Method
Journal Title: Journal of Microbiology and Infectious Diseases - Year 2018, Vol 8, Issue 3
Abstract
Antifungal Susceptibility Pattern among Candida species: An Evaluation of Disc Diffusion and Broth Micro-dilution Method Abirami Lakshmy Jayachandran1, Radhika Katragadda2, Thyagarajan Ravinder2, Leela Vajravelu2, Lavanya Manorajan2, S. Hemalatha2, Kaliappan Shanmugam3 1Department of Microbiology, Karpagavinayaga Institute of Medical Sciences and Research Center, Kanchipuram, Tamil Nadu, India 2Department of Microbiology, Government Kilpauk Medical College and Hospital, Chennai, Tamil Nadu, India 3Dharmapuri Government Medical College, Tamil Nadu, India ABSTRACT Objective: Candida species are emerging as a significant pathogen. Certain species of Candida like Candida krusei are inherently resistant to azoles. In vitro susceptibility testing is essential for guiding therapy. The present study aims to study the antifungal susceptibility pattern of Candida isolates by Disc Diffusion and Broth Micro-dilution method and to evaluate the degree of agreement between both the techniques. Methods: Candida isolated from specimens like Oropharyngeal swabs, blood, pus and wound swabs were included in the study. Speciation was done as per standard microbiological methods. Antifungal resistance was determined by Disc Diffusion method for Fluconazole, Itraconazole, Amphotericin B, Nystatin and Clotrimazole. Broth Micro-dilution method was performed for Fluconazole, Itraconazole and Amphotericin B. The degree of agreement between both the methods for the resistant isolates was analyzed by deriving the kappa value. Results: Out of the 156 Candida isolates obtained, Candida albicans was the most common species isolated. By Disc Diffusion method Fluconazole and Itraconazole showed an overall resistance of 34 (21.7%) and 27 (17.3%), respectively. Using Broth Micro-dilution method, Fluconazole and Itraconazole had a resistance percentage of 35 (22.4%) and 29 (18.5%), respectively. There was a good agreement between both the methods in detecting the percentage of resistant isolates for Fluconazole and Itraconazole (κ=0.9). Conclusion: It is essential to perform susceptibility testing for all the Candida isolates for providing crucial information about the resistance pattern and help in choosing the appropriate antifungal drug for therapy. Disc diffusion method which is easy to perform can be utilized for day to day practice. J Microbiol Infect Dis 2018; 8(3):97-101
Authors and Affiliations
Abirami Lakshmy Jayachandran, Radhika Katragadda, Thyagarajan Ravinder, Leela Vajravelu, Lavanya Manorajan, S. Hemalatha, Kaliappan Shanmugam
Keywords
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- EP ID EP482071
- DOI 10.5799/jmid.458457
- Views 121
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