ANTIMICROBIAL ACTIVITY AND POTENCY OF CASSIA ABBREVIATA OLIV STEM BARK EXTRACTS
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2015, Vol 7, Issue 6
Abstract
Objective: To evaluate the potential antimicrobial activity and relative potency of aqueous and organic extracts of Cassia abbreviata Oliv stem bark against clinical isolates of Neisseria gonorrhoeae (NG), Pseudomonas aeruginosa (PA), Klebsiella pneumoniae (KP) and Candida albicans (CA).Methods: Six extracts of Cassia abbreviata Oliv stem bark were prepared as follows: Four extracts were prepared following Soxhlet extraction procedure using ethanol, water, trichloromethane (TCM) and dichloromethane (DCM)+ethanol (1:1) as solvents, respectively. Two extracts were prepared by soaking the powdered stem bark in ethanol and water, respectively, and subjected to mechanical shaking for 8 hours. Antimicrobial activities and minimum inhibitory concentrations (MIC) of extracts were evaluated in-vitro using agar well diffusion assay.Results: All extracts except TCM were active against NG and PA with an average MIC of 78.8 µg/ml. The cold aqueous extract had a lowest MIC (46.88 µg/ml) against PA, whereas both hot and cold ethanol extracts had a lowest MIC of 46.88 µg/ml against NG. Only hot extracts (ethanol, DCM+ethanol and TCM) were active against KP with a lowest MIC of 46.88 µg/ml of TCM extract. Both extracts of cold ethanol and DCM+ethanol were active against CA with cold ethanol extract having a lower MIC of 93.75 µg/ml compared with DCM+ethanol.Conclusion: There were variations in in-vitro antimicrobial activities (microbial growth inhibition) of Cassia abbreviata Oliv stem bark extracts which depended on the solvent used. Organic extracts were more potent against NG, PA, KP and CA compared with aqueous extracts.Â
Authors and Affiliations
Mwila Mulubwa, Shiv Prakash
Keywords
Related Articles
- EP ID EP578735
- DOI -
- Views 64
- Downloads 0
BIOCONTROL OF FUSARIUM OXYSPORUM IN UNSTERILIZED SOIL BY NOVEL STREPTOMYCES CACAOI SUBSP CACAOI [M20]
Objective: To find bio fungicide from mangrove actinomycetes for controlling seed and soil borne pathogen-Fusarium oxysporum. Methods: A total of 25 actinomycetes were isolated by pour plate method. These were screened f...
ENANTIOMERIC SEPARATION OF ETODOLAC IN A BULK DRUG SUBSTANCE BY REVERSE-PHASE CHIRAL LIQUID CHROMATOGRAPHY METHOD
Objective: To develop novel, simple and rapid enantiomeric separation of Etodolac by reverse-phase high-performance liquid chromatographic method as per ICH guidelines.Methods: The R-isomer and S-isomer were baseline res...
IN VITRO ANTIOXIDANT ACTIVITY AND GC-MS ANALYSIS OF THE ETHANOLIC EXTRACTS OF TERMINALIA BELLERICA ROXB (BAHEDA)
Objective: To investigate the antioxidant activity of ethanolic extract of Terminalia bellerica fruit pulp (TBFP), seed (TBS) and bark (TBB), and identification of phytochemical constituents of said extracts.Methods: The...
EFFECT OF LACOSAMIDE ON BEHAVIOUR OF CHILDREN WITH REFRACTORY PARTIAL EPILEPSY
Objective: A unicentre, prospective study was carried out to investigate the behavioral effects of lacosamide as adjunctive therapy with refractory partial epileptic children in tertiary care hospital with prior approval...
HYPOTENSIVE ACTIVITY OF THYMOQUINONE IN NORMOTENSIVE RATS AND ITS RECEPTOR MECHANISMS
Objective: The objectives of the current study were to confirm the blood pressure lowering effect of thymoquinone (TQ) and to investigate its mechanism through muscarinic and β-adrenergic receptors.Methods: Mean arteria...