ANTIMICROBIAL ACTIVITY AND TLC PROFILING OF CLERODENDRUM PHLOMIDIS LINN. F LEAF EXTRACT AGAINST MULTI-DRUG RESISTANT CLINICAL PATHOGENS
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2017, Vol 9, Issue 9
Abstract
Objective: The aim of the present study is to evaluate the antibacterial and antifungal activities of different solvent extracts of leaves of Clerodendrum phlomidis against multi-drug resistant clinical pathogens and to perform Thin Layer Chromatography (TLC) profiling of the extracts.Methods: The leaf powder was sequentially extracted with petroleum ether, ethyl acetate, acetone, and methanol. The extracts were tested for their antimicrobial activity against 20 bacterial strains and 15 fungal strains which were multiple drugs resistant by disc diffusion method. TLC profiling of all four extracts was performed.Results: The petroleum ether and ethyl acetate extracts showed antimicrobial activity against four bacteria and seven fungal strains. The highest zone of inhibition (18±0.50 mm) for bacteria was recorded against Staphylococcus epidermis by ethyl acetate extract and for fungi, the petroleum ether extract showed 13±0.00 mm against Trichophyton rubrum. TLC profiles showed that ethyl acetate extract possessed more compounds.Conclusion: From the results of the present study it is concluded that the petroleum ether and ethyl acetate extracts of C. phlomidis leaves are good sources of antimicrobial compounds which can inhibit multiple drug resistant pathogens.
Authors and Affiliations
A. Hannah Hepsibah, M. Mala, G. Jeya Jothi
Keywords
Related Articles
- EP ID EP575529
- DOI 10.22159/ijpps.2017v9i9.20186
- Views 55
- Downloads 0
SEPARATION AND ASSAY OF FOUR ANTIHISTAMINE DRUGS DIPHENHYDRAMINE, CHLORPHENIRAMINE, CYPROHEPTADINE AND FEXOFENADINE IN PHARMACEUTICAL FORMS BY A SINGLE HPLC METHOD
Objective: The objective of this study was to develop and validate a single HPLC method, in order to separate and assay four antihistamine drugs diphenhydramine, chlorpheniramine, cyproheptadine and fexofenadine in pharm...
SIMULTANEOUS DETERMINATION OF MOXIFLOXACIN HYDROCHLORIDE AND DIFLUPREDNATE BY RATIO DERIVATIVE SPECTROPHOTOMETRY
Objective: Ratio derivative spectrophotometric method has been developed for the simultaneous determination of Moxifloxacin HCl (MH) and Difluprednate (DIFLU) in Phosphate Buffer pH 7.4.Methods: In this method, the overl...
ADULTICIDAL ACTIVITY OF BOTANICAL OILS BY IMPREGNATED PAPER ASSAY AGAINST CULEX QUINQUEFASCIATUS SAY
Objective: The present study was undertaken with the aim of finding out the efficacy of essential oils (EOs) as anti-mosquito agents for commercial purposes. Plant source insecticides as an alternative to chemical insect...
IMPLEMENTING SUSTAINABLE ERGONOMICS FOR POWER-LOOM TEXTILE WORKERS
Objective: This ergonomic study focuses on identifying and understanding the occupational health and safety risk aspects of power-loom industry workers from Solapur City, Maharashtra, India.Methods: The samples of 540 wo...
DIALYSIS PRACTICE PATTERN IN PATIENTS UNDERGOING HEMODIALYSIS IN A TERTIARY AND A PRIVATE HOSPITAL
Objective: To evaluate the dialysis practice pattern in chronic kidney disease patients undergoing hemodialysis in a tertiary and a private hospital Methods: A prospective observational study of six months duration was...