Antimicrobial Activity of Bauhinia Purpurea (L) by Minimum Inhibitory Concentration (MIC) Method
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2017, Vol 12, Issue 1
Abstract
MIC methods are widely used in the comparative testing of new agents, or when a more accurate result is required for clinical management. As there are no CLSI (formerly NCCLS) recommendations for the determination of MICs of varies bio-compounds against gram positive and gram negative organisms. The minimum inhibitory concentration is the lowest concentration (in μg/ml) of an antibiotic that inhibits the growth of a given strain of bacteria. In Diagnostic laboratories these MIC’S are used to confirm resistance and also most often used to determine antimicrobial invitro activity. In the present MIC method, one can get the information about Antibiotic agent, preparation of stock solutions, media and inoculation, conditions of incubation, ultimately reading and interpretation of results. The present study is focused to evaluate antibacterial activities of ethanolic extract and purified fractions of Bauhinia purpurea using a gram-positive and a gram-negative organisms Staphylococcus aureus , Klebsiella, E.coli and enterococcus.
Authors and Affiliations
1*Shamala T, A. M. Krupanidhi, Shanmukhappa S,, B. E . Basavarajappa, Jayamma kulkarni
Keywords
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- EP ID EP388206
- DOI 10.9790/3008-1201026971
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