Antimicrobial Activity Of Murraya Koenigii And Zingiber Officinale Plants On Drug Resistant Pathogens Isolated From Clinical Specimens And Preliminary Phytochemical Analysis Of The Crude Extract Preparations.
Journal Title: IOSR Journal of Biotechnology and Biochemistry (IOSR-JBB - Year 2018, Vol 4, Issue 3
Abstract
The aim of the present study was to investigate the antimicrobial activity and phytochemical screening of Murraya koenigii and Zingiber officinale plant extracts. Ethanol and Methanol were used as the solvents for extraction. The antimicrobial activities of these plant extracts were tested against the drug resistant Gram-positive and Gram-negative bacterial strains. The bacteria used in the study were Staphylococcus aureus, Staphylococcus epidermidis, Acinetobacter baumannii, Salmonella spp., Proteus mirabilis, Escherichia coli, Pseudomonas aeruginosa, Enterococcus faecium and Neisseria spp. The ethanol and methanol extracts of leaves of Murraya koenigii and rhizome extracts of Zingiber officinale showed promising results against the drug resistant pathogens with zone diameter of inhibition more than 15mm. The ethanol extracts gave larger zones of inhibition than methanol extracts, suggesting that ethanol is a better solvent in terms of extraction of phytochemicals from the plants. It was also observed from the results that the extracts worked better towards the inhibition of gram positive bacteria Staphylococcus aureus and Staphylococcus epidermis than the gram negative bacteria used in the study. The Minimum Inhibitory Concentration was determined for the extracts which gave a zone diameter of inhibition of 15mm or more than 15mm to suggest the minimum therapeutic dose that can inhibit the test pathogens. The Preliminary Phytochemical Analysis revealed the presence of various phytochemicals like Alkaloids, Steroids, Flavanoids, Terpenoids, Reducing Sugars, Glycosides, Tannins, and Phenols in different extracts analyzed. Hence, it could be suggested the antibacterial activity observed in the different extracts tested could be due to the presence of these phytochemicals.
Authors and Affiliations
Nagesh Malik, Samreen Ahmed
Keywords
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- EP ID EP415093
- DOI 10.9790/264X-0403010510.
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