Isolation of Host Specific Bacteriophages against Salmonella and Methicillin Resistant Staphylococcus aureus (MRSA) From Hospital Waste Water
Journal Title: IOSR Journal of Biotechnology and Biochemistry (IOSR-JBB - Year 2018, Vol 4, Issue 4
Abstract
Worldwide emergence of new resistance mechanisms in constantly increasing infections of Multidrug Resistance (MDR) bacteria (such as Methicillin resistant Staphylococcus aureus (MRSA) and Salmonella) is generating a major problem in treatment or prevention of infection against these pathogens. Development of resistance reduces the effectiveness of antibiotics against them. Antibiotics are costly and also harm useful microbes of host body, thus there is a need to adopt drug-independent remedial strategies to defeat pathogens. Phage therapy has therefore been considered as one of the strongest alternatives to antibiotics. Host specific Bacteriophages naturally and specifically kill their host without disturbing the normal microbes of host organism also provides cost effective treatment. Present Study describes the isolation of MRSA and Salmonella specific Bacteriophages from hospital sewage water sampled from sewage treatment plant at local hospital in jaipur and in vitro analysis of their lytic efficiency by plaque assay. Bacterial host one MRSA strain and two Salmonella typhi strain for this experiment collected from Sawai Man Singh hospital jaipur. Successful isolation of host specific Bacteriophages and determination of their lytic efficiency support the evidence of phage therapy as promising approch againt multidrug resistant pathogenic hosts bacterium in present also since it started and hope to take world advantage and application of bacteria and phage relation to treat MDR infections naturally as much as possible.
Authors and Affiliations
Pratibha Goyal, Dr. Nupur Mathur, Dr. Anuradha singh
Keywords
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- EP ID EP415116
- DOI 10.9790/264X-0404011115.
- Views 158
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