Antimicrobial and Phytochemical Evaluation of Calotropis procera (“SODOM APPLE”) against Human Pathogens
Journal Title: Microbiology Research Journal International - Year 2016, Vol 11, Issue 3
Abstract
Aim: To evaluate the antimicrobial and phytochemical effects of acetone, ethanol, methanol and aqueous leaf extracts of Calotropis procera on human pathogens. Study Design: Five pathogenic and two fungi species were obtained from the Department of Biological Sciences, Afe Babalola University, Ado-Ekiti and were evaluated in in vitro antibacterial testing. Methodology: We studied the in vitro antimicrobial sensitivity of C. procera by well in agar diffusion method. Also studied was the extract durability to ascertain expiration after preparation and the phytochemical constituents of the extracts by chemical methods. Results: The results revealed that acetone extract exhibited the highest antimicrobial properties on the test organisms followed by methanol, ethanol and aqueous extracts in that order. However, Morganella morgani was the most inhibited by the solvent extracts with zone of inhibition 45, 56, 59 and 43 mm by acetone, methanol, ethanol and aqueous extracts, respectively. The minimum inhibitory concentration (MIC) of acetone extract on bacteria species was between 25.0 and 100 mg/ml and between 25 and 50 mg/ml on the fungal species. Minimum bactericidal and fungicidal concentrations (MBC/MFC) of the extracts were valued at concentrations ranged from 50-100 mg/ml on the selected microorganisms. The durability study of the leaf extracts in consistent sensitivity pattern was potentially effective for 57-66 days. Phytochemical analysis of the leaf extract showed the presence of saponins, alkaloids, tannins, steroids, tarpenoids, flavonoids, phenolics and carotenoids. The results provide a partial support for the use of C. procera in traditional medicine.
Authors and Affiliations
Asoso, Oluwakemi Oyesola, Akharaiyi, Fred Coolborn, Oladunmoye, Muftau Kolawole, Makinwa, Bisola
Keywords
Related Articles
- EP ID EP350749
- DOI 10.9734/BMRJ/2016/16372
- Views 91
- Downloads 0
Isolation, Screening and Identification of Biosurfactant-producing Bacteria from Hydrocarbon-polluted and Pristine Soils within Ogoniland, Nigeria
Aim: This study investigated the production of biosurfactant from bacteria isolated from hydrocarbon-polluted and pristine soils within Ogoniland in the Niger Delta region of Nigeria. Methods: Baseline physicochemical p...
Epidemiology, Phenotyping and Antimicrobial Susceptibility Profile of Enterohaemrrhagic Escherichia coli Strains Isolated from Cases of Diarrhea
Aim of the Work: this work aimed to study the Prevalence, Epidemiology, Phenotyping, and Antimicrobial susceptibility profiles of Enterohaemrrhagic Escherichia coli (EHEC) strains among cases of infantile and childhood d...
A New Nomenclature for Cry1Ab Proteins Reflecting 3-D Structure Differences
Cry1Ab proteins produced by the insecticidal bacterium Bacillus thuringiensis are mostly studied and applied, facing the challenge of insect resistance. The 3-D structure of the toxic core for all available 34 Cry1Ab pro...
Detection of Microbial Contamination of Processed Beef Meat by Using API Strips and Automated Vitek 2 Compact System
Aims: Food safety is a complex issue, where animal proteins such as meats, meat products, and fish and fishery products are generally regarded as high risk commodity with pathogenic microrganisms. The present study was c...
Physicochemical and Microbiological Qualities of Water and Soil Samples from Groundnut Oil Producing Industry
Triplicate tap and wastewater samples collected from a groundnut oil producing industry in Akure metropolis, Southwest Nigeria were analysed for physicochemical and microbiological qualities using standard procedure. Adj...