Detection of Microbial Contamination of Processed Beef Meat by Using API Strips and Automated Vitek 2 Compact System
Journal Title: Microbiology Research Journal International - Year 2016, Vol 13, Issue 2
Abstract
Aims: Food safety is a complex issue, where animal proteins such as meats, meat products, and fish and fishery products are generally regarded as high risk commodity with pathogenic microrganisms. The present study was conducted to evaluate microbial contamination of beef meat products; minced meat, sausage, beef burger and Shawerma in supermarkets and cafeterias in Khartoum towns. Study Design: Fifteen samples of minced meat, 12 samples of beef burger, 12 samples of sausage and 12 samples of Shawerma were collected randomly at Khartoum State (Khartoum, Omdurman and Bari towns markets) during the period from October 2011-August 2012 and examined for bacterial contamination. Methodology: Primary culturing of the samples were conducted onto blood agar plates and the purified isolates of the Gram-Negative bacteria were identified by rapid biochemical tests (Api 20 E, Api 20 NE) while Gram-Positive bacteria were identified by Api Staph and automated system (Vitek 2 compact) according to manufacturer’s instructions. The Bacillus spp. was identified by conventional bacteriological methods. Results: One hundred and two different bacteria were isolated and identified from 51 beef meat products. The most Gram- Positive isolates were Lactobacillus spp. 11(10.7%), and Kocuria kristinae 9(8.8%) while the most Gram-Negative isolates were Proteus mirabilis 7(6.8%) and Klebsiella pneumoniae 7(6.8%). Conclusions: This study revealed that microbiological quality of beef meat products is strongly influenced by implementation of hygienic precautions during production and handling. The environment in butcher shops and areas of production can acts as important source of microbial contamination.
Authors and Affiliations
Awatif A. Ahmed, Y. A. Sabiel
Extended-Spectrum -Lactamase-Producing Salmonella Species Isolated from Diarrhoeal Patients in Bangladesh: Characterization and Their Dissemination through Conjugation
Objective: Infections with Salmonella resistance to extended-spectrum cephalosporins threaten the efficacy of drugs for treating samonellosis. So the aim of this study was to characterize the ESBL (Extended Spectrum β-la...
Antibacterial and Antioxidant Potential of Leaf and Seed Extracts of Murraya koenigii (Linn.) Spreng
India is known for its rich biodiversity and traditional healers have used flora to cure various human ailments. The same plants are still being used today for the benefit of mankind. Since the beginning of this century,...
Evaluation of Antimicrobial Potency and Phytochemical Screening of Persea americana Leaf Extracts against Selected Bacterial and Fungal Isolates of Clinical Importance
Aim: The quest for novel bioactive from natural sources informed the evaluation of the antimicrobial alongside the phytochemical composition of leaf extracts of Persea americana obtained from Akure, Ondo State, Nigeria....
Evaluation of Microbial Availability and Linkages to the Physicochemical Quality of Natural and Deteriorated Rubber Latexes
Natural and deteriorated rubber latex samples were analyzed for bacteriological and physicochemical quality. Bacterial isolates obtained after 24 hours of incubation ranged from 1.5x106 to 1.58x108 CFU/mL, corresponding...
Epidemiology of Carbapenem Resistance among Multi-drug Resistant Enterobacteriaceae in Uganda
Background: Multi-drug resistant (MDR) Enterobacteriaceae are on the increase worldwide and their spread has become a global challenge. Escalating the challenge is the possibility that many of these are Carbapenemase-pro...