Mycodegradation of Crude Petroleum Oil by Locally Marine Candida tropicalis

Journal Title: Microbiology Research Journal International - Year 2017, Vol 21, Issue 6

Abstract

Aims: A great demand for crude oil as a source of energy has resulted in an increase in its production, which in turn a gross pollution of the environment. In this study, isolation of local marine yeast with a high potency in petroleum oil biodegradation was the target. Place and Duration of Study: Samples were collected from oil-spilled seawater in Shalateen, Red Sea, Egypt. Methodology: Redox indicator 2, 6-dichlorophenolindophenol (DCPIP) and oil extract dichloromethane were used for biodegradable crude oil verification. GC-MS analysis of the residual degraded oil was carried out during the biodegradation process. Biotoxicity of residual oil extracts was performed at interval time (0, 6th and 12th day). Bioaugmentation process was accomplished to imitate application on polluted site and determine biodegradable oil activities for both amended and non- amended polluted water samples (either under sterile or non-sterile conditions). Results: Local marine Candida tropicalis strain was isolated and identified using partial sequence of 18sRNA gene. C. tropicalis was significantly (p < 0.05) capable to remove 98.63±0.73% crude oil (50% v/v) after 12 days of incubation at 25° C. Using GC-MS analysis, marine yeast isolate was capable of degrading the aliphatic fractions in crude oil, where almost hydrocarbons peaks were consumed during the degradation process. Using Artemia salina as a biomarker to test the toxic effect of residual oil extracts at interval time (0, 6th and 12th day), compared with the blank and control test, revealed that no-toxic intermediates or end products were detected. Non-sterile non-amended culture significantly (p < 0.05) showed 94.23±0.93% oil removal followed by non-sterile amended culture 65.5±7.7%. Conclusion: These data demonstrated that the prospect in protecting the environment from oil pollution achieves after the application of marine C. tropicalis isolate.

Authors and Affiliations

Khouloud M. I. Barakat

Keywords

Related Articles

Ring Worm Skin Infection in a Rural Community of Bushenyi (Uganda)

Aim: Ring worm skin infections which have continuously been neglected in several communities of sub Saharan Africa, thus leading to the development of herbal remedies as communities seek to find solutions to the menace....

Evaluation of in vitro Anti-proliferative Activity of L-arginine deiminase from Novel Marine Bacterial Isolate

L-Arginine deiminase is a therapeutic l-arginine depleter found to counteract various arginine auxotrophic cancer cells (do not express ASS/OCT). The aim of the present study was to evaluate the anti -proliferative activ...

Characterization of Plant Growth Promoting Rhizobacteria Isolated from Chickpea (Cicer arietinum)

Aims: To isolate bacterial strains from chickpea rhizospheric soil and nodules, to characterize and identify potential bacterial strains by using 16S rRNA gene sequencing. Place and Duration of Study: Department of Soi...

Traditional and Molecular Identification of Bacterial Contamination from Honey (Processed and Unprocessed) and Their Control by Phytotherapy

Honey used as a potential source of medicine traditionally. Today honey is contaminated due to improper handling which leads to cause various infections. So, the aim of current study was to isolate and identify the bacte...

Antimicrobial Activity of Moringa oleifera Leaf against Isolates of Beef Offal

This study was designed to evaluate the antimicrobial potentials of ethanol and aqueous extracts of Moringa oleifera against microorganisms isolated from beef offal. The beef offal samples (liver, kidney and intestine) w...

Download PDF file
  • EP ID EP316073
  • DOI 10.9734/MRJI/2017/37082
  • Views 100
  • Downloads 0

How To Cite

Khouloud M. I. Barakat (2017). Mycodegradation of Crude Petroleum Oil by Locally Marine Candida tropicalis. Microbiology Research Journal International, 21(6), 1-10. https://europub.co.uk/articles/-A-316073