Antimicrobial Assessment of Fresh Ripe and Dry Ripe Musa sapientum L. Peels against Selected Isolates Associated with Urinary Tract Infection in Port Harcourt, Nigeria
Journal Title: Journal of Medical Science And clinical Research - Year 2018, Vol 6, Issue 12
Abstract
Banana peels is the outer coverage of the banana fruits commonly used to feed animals, fish and many others or thrown away as waste. Almost every part of a banana plant has medicinal values. Urinary Tract Infection is an infection that affects any parts of your urinary system (upper or lower). Usually its treatment and diagnosis are usually with antibiotics urine sample. Increase in bacterial resistant to conventional antibiotics has prompted the development of bacterial disease treatment strategies that are alternatives to conventional antibiotics. Aim: This study is to assess the antimicrobial properties of banana peels against selected isolates from Urinary Tract Infection sample. Methods: A Mid- stream urine samples collected from patients visiting BMSH with suspected cases of UTIs, were cultured. The isolates from culture was further analysis with agarose gel electrophoresis for the presence of 16SrRNA and Phylogenetic analysis shows Staphylococcus sciuri strain, a coagulase‐negative species, Escherichia coli, Enterococcus faecalis, Klebsiella pneumoniae and Proteus mirabilis. Fresh ripe and dry ripe were used to determine the antimicrobial activities. A measure of 150grams of both fresh ripe and dry ripe was extracted with 100ml of both 70%ethanol and water solvent (sterile) respectively for about 2 days. The solvent extracts were concentrated separately under reduced pressure, 10g of each concentrated solvent extracts were dissolved in 5ml of sterile distilled water and used for antimicrobial assay using agar well diffusion method. Results: The phytochemical analysis of fresh ripe and dry ripe shows that alkaloid, flavonoid, cardiac glycosids, phenols and tannins were present. The zone of inhibition recorded from the extracts on selected organism was concentration dependent (100%, 80%, 60%, 40% and 20%). The higher the concentration the wider effect on each of the test isolates. Musa sapientum L peels showed some effect on Escherichia coli, Enterococcus faecalis, Staphylococcus sciuri, Klebsiella pneumoniae and Proteus mirabilis at 100% and few at 80% concentrations for both solvents. Dry ripe has advantage over fresh ripe banana peels and the type of solvent (ethanol) also play a synergetic effect. The peels of Musa sapientum exhibited some inhibitory activity on these selected UTIs isolates, could be attributed to the presence of certain secondary metabolites. Conclusion: Finally, when compared the peels extracts against the standard antibiotics drugs as my control, the water solvent extract were less effective compared to the ethanoic extracts, which were also smaller in size of their zone of inhibition.
Authors and Affiliations
Konne, Felix Eedee
Keywords
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