Antimicrobial Compound from Streptomyces Isolate Characterized Using HPLC
Journal Title: Universal Journal of Environmental Research and Technology - Year 2012, Vol 2, Issue 4
Abstract
Need for novel, safe and more efficient antibiotics is a key challenge to the pharmaceutical industry today. The ever increasing knowledge in the area of pathogen`s drug resistance has evoked the discovery of new antibiotics by the screening of microbes. Last few decades has witnessed the production of novel antibiotics from different microorganisms. At present, aerobic Actinomycetes have attracted considerable attention of bacteriologist, geneticist and ecologist because of the production of novel antibiotics. In this research we evaluate the potential of antibiotic production and characterize HPLC (High performance liquid chromatography) analysis pattern of Streptomyces from various semi-arid locations of Jaipur, Rajasthan. Regarding this, five soil samples were collected randomly from three different green cover areas of Jaipur. Then, following the extraction of secondary metabolite, the HPLC analysis was carried out for characterization of various extracts. Considering the coordinate analysis of HPLC pattern, isolate A4 was found to be a potential producer of an antibiotic ‘Monensin’. The results highlight the importance of Streptomyces isolates in antibiotic and antifungal production. HPLC confirmed the production when compared with standards.
Authors and Affiliations
Nupur Mathur, Anoop Paliwal, Pratibha Sharma, Manish Kumar, Pradeep Bhatnagar
Keywords
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