ANTIOXIDANT AND ANTI-LIPIDPEROXIDATION POTENTIALS OF THE ETHYLACETATE AND CHLOROFORM EXTRACTS OF BASELLA ALBA LEAVES
Journal Title: ASIAN JOURNAL OF NATURAL & APPLIED SCIENCES - Year 2013, Vol 2, Issue 2
Abstract
The search for antioxidants in various plants fractions became pertinent since they limit the oxidation of macro molecule by free radical that leads to cellular oxidative damage implicated in several chronic human diseases. The present study was undertaken to find the antioxidant and inhibitory potentials of lipid peroxidation by ethyl acetate and chloroform extracts of Basella Alba leaves. Extracts were prepared by soaking dried leaves of the selected plant in appropriate solvents using liquid-liquid chromatographic technique. Phenol and Flavonoid contents of the extracts were measured by Folin Ciocalteu and Aluminium chloride assays. Hydroxyl radicals and 1,1diphenyl 2-picrylhydrazyl (DPPH) scavenging effect were determined using deoxyribose assay and the ability of the extract to donate electron to the stable DPPH. Inhibition of lipid peroxidation was carried out using the reactions of peroxides with Thiobarbituric acid. Results revealed the total phenolic contents equivalent of gallic acid (GAE) of the ethyl acetate and chloroform extracts were 0.029±0.001mg/g and 0.030± 0.002mg/g respectively. The total flavonoids content, equivalent of quercetin standard were 0.045±0.001mg/g and 0.085±0.001mg/g. The extracts exhibited radical scavenging activity in a concentration dependent manner. Ethylacetate and chloroform extract scavenged DPPH radical by 57.29% ±0.011 and by 82.19%±0.01 at200µ g/mL concentrations. Maximum hydroxyl radical scavenging activity was obtained at 150µg/mL by ethylacetate (50.10±0.1%) and chloroform (41.10%) extracts respectively. The two fractions conferred 50% protection at maximum concentration on lipid peroxidation induced by FeS04 , as inhibition was dose dependent. Results obtained shows that certain bioactive agents with antioxidant and anti-lipidperoxidation properties are present in the leaves extracts. This study give scientific support for its wide consumption as leafy vegetable in western part of Nigeria as a rich source of dietary antioxidant, while these extracts may be explored as template in drug discovery and design especially in prevention and management ofdiseases associated with aging.
Authors and Affiliations
Olaniyi T. Adedosu, Tolulope A. Oyedeji. , Olusegun K. Afolabi, Adeniran S Adekunle, Adebayo L. A dedeji
Keywords
Related Articles
- EP ID EP104097
- DOI -
- Views 94
- Downloads 0
IN VITRO ANTIMALARIAL ACTIVITY OF THAI PICRASMA JAVANICA Bl STEM EXTRACT
The in vitro antimalarial activity against Plasmodium falciparum K1 of the four extracts from the stem of Picrasma javanica Bl, that is, water, methanol, chloroform and hexane were studied using a modification of the [3H...
VULNERABILITY OF WATER INFRASTRUCTURE TO TERRORISM
Terrorists groups like Al-Shabaab and Al-Qaeda have the knowledge and the capability to strike anywhere in Kenya and with Kenya Defence Forces (KDF) incursion into Somalia in mid-October 2011, the citizens in Moy...
Restricted Activation Via SMS For Mobile Contracting Service
Before the Information and Communication Technology (ICT), the old traditional security was managed by human that is very easy to handle even inaccurate, expensive, and unreliable. Furthermore, it is very changeable from...
ENERGY AND PERFORMANCE MODELING OF DATAFLOW APPLICATIONS ON MANY-CORE ARCHITECTURES
Many-core processors will provide system developers on embedded platforms with the best means of achieving system flexibility, shorter time to market, longer device life cycles and overcome the hardware productivit...
ISOLATION OF MORIN FROM THE WOOD OF THAI ARTOCARPUS HETEROPHYLLUS
The wood of Artocarpus heterophyllus wildly used in Thailand for medicinal purposes included good health and tonic. One active compound that response for these purposes shall be the antioxidant morin (2/,3,4/,5,7-pentahy...