Antioxidant and Free Radical Scavenging Potential of Allium hookeri Thwaites Roots Extract Studied Using In Vitro Models
Journal Title: JOURNAL OF ADVANCES IN BIOLOGY - Year 2014, Vol 4, Issue 1
Abstract
Aim; To study antioxidant and free radical scavenging potential of Allium hookeri methanol roots extract (MREAH) using different in vitro models Materials and Methods; Different in vitro models such as lipid peroxidation, metal chelation and reducing potential assays were used to determine antioxidant potential and 1, 1-diphenyl-2-picryl-hydrazil (DPPH,) superoxide (O2.-), hydrogen peroxide (H2O2), nitric oxide (NO.) and hydroxyl radical (HO.) radical scavenging assays for free radical scavenging abilities of MREAH. Results; MREAH recorded concentration dependent effect on inhibition of lipid perodixation (IC50= 90.15 ± 1.20μg/ml), effective metal chelation (IC50=62.45 ± 1.27μg/ml) and higher reducing potential (ODmax=1.12 ± 0.20). MREAH could efficiently scavenged DPPH (IC50 = 58.23±1.39μg/ml), O2.- (IC50=140.30 ± 2.49μg/ml), H2O2 (IC50=123.86 ± 3.00 μg/ml), NO . (IC50 = 86.36 ± 2.01 μg/ml) and HO. (IC50 = 90.10 ± 1.80 μg/ml) radicals in dose dependent manner. The presence of polyphenol, flvanoids and ascorbic acids in the amounts 40.60 ± 1.10 mg/ml gallic acid equivalent-polyphenols and 28.44 ± 0.90 mg/ml quercetin equivalent-flavanoids and 24.20 ± 1.10 mg/ml ascorbic acid respectively may be attributed for these activities obtained per se in MREAH Conclusion; These observations reveal antioxidant and free radical scavenging potentials of MREAH that support their therapeutic used with consequent health benefits.
Authors and Affiliations
Kshetrimayum Birla Singh, Nongmaithem Mohondas Singh
Keywords
Related Articles
- EP ID EP654381
- DOI 10.24297/jab.v4i1.1940
- Views 188
- Downloads 0
Yersinia enterocolitica survival in aquatic environment: epidemiological significance
The survival of four biotypes of Yersinia enterocolitica (2 isolated from swine and 2 from Chironomus larvae) has been assessed in two types of surface water, with low and high content of organic matter, at 3 different t...
EVALUATION OF THE ANTICANDIDOSIC ACTIVITY OF THE CRUDE AQUEOUS AND ETHANOLIC EXTRACT OF EUCALYPTUS SP, A MYRTACEE FROM THE IVORIAN PHARMACOPEIA ON THE IN VITRO GROWTH OF CANDIDA ALBICANS, CANDIDA GLABRATA and CANDIDA TROPICALIS
With the advent of AIDS/ HIV, we assist to a trong new outbreak of thrive's mycoses. To fight against this mycoses apparition, our research team has tested the whole crude extracts action (water and hydro-alcoholic), out...
Efficiency Economic Of Chainsaw and Timber Jack 450C Skidder Evaluation in Felling Operation and Ground-Base Skidding System
In this research, an empirical time study was carried out, to estimate effects of a tree felling and skidding operations on time consumption, productivity rate, cost, and present a regression model of chainsaw and Timber...
OsNPR1 GENE EXPRESSION IN RESPONSE TO SALYSILIC ACID IN RICE
NPR1 gene plays an important role in the activation of systemic acquired resistance (SAR). This research was conducted to examine the expression profiles of OsNPR1 gene in four rice varieties: Pongsu Seribu, MR219, MRQ74...
ISOLATION, CHARACTERISTICS, AND ANTIOXIDANT ACTIVITY OF LOW MOLECULAR COMPOUNDS OF FRUIT BODIES LACTARIUS PERGAMENUS (Fr.) Fr MUSHROOMS
Methanol and methylene chloride extracts were obtained from fresh and dried fruit bodies of Lactarius pergamenus (Fr.) Fr. and studied by gas-chromatography mass spectrometry (GC/MS), thin-layer chromatography (TLC), and...