Antioxidant and Free Radical Scavenging Potential of Allium hookeri Thwaites Roots Extract Studied Using In Vitro Models
Journal Title: JOURNAL OF ADVANCES IN BIOLOGY - Year 2014, Vol 4, Issue 1
Abstract
Aim; To study antioxidant and free radical scavenging potential of Allium hookeri methanol roots extract (MREAH) using different in vitro models Materials and Methods; Different in vitro models such as lipid peroxidation, metal chelation and reducing potential assays were used to determine antioxidant potential and 1, 1-diphenyl-2-picryl-hydrazil (DPPH,) superoxide (O2.-), hydrogen peroxide (H2O2), nitric oxide (NO.) and hydroxyl radical (HO.) radical scavenging assays for free radical scavenging abilities of MREAH. Results; MREAH recorded concentration dependent effect on inhibition of lipid perodixation (IC50= 90.15 ± 1.20μg/ml), effective metal chelation (IC50=62.45 ± 1.27μg/ml) and higher reducing potential (ODmax=1.12 ± 0.20). MREAH could efficiently scavenged DPPH (IC50 = 58.23±1.39μg/ml), O2.- (IC50=140.30 ± 2.49μg/ml), H2O2 (IC50=123.86 ± 3.00 μg/ml), NO . (IC50 = 86.36 ± 2.01 μg/ml) and HO. (IC50 = 90.10 ± 1.80 μg/ml) radicals in dose dependent manner. The presence of polyphenol, flvanoids and ascorbic acids in the amounts 40.60 ± 1.10 mg/ml gallic acid equivalent-polyphenols and 28.44 ± 0.90 mg/ml quercetin equivalent-flavanoids and 24.20 ± 1.10 mg/ml ascorbic acid respectively may be attributed for these activities obtained per se in MREAH Conclusion; These observations reveal antioxidant and free radical scavenging potentials of MREAH that support their therapeutic used with consequent health benefits.
Authors and Affiliations
Kshetrimayum Birla Singh, Nongmaithem Mohondas Singh
Keywords
Related Articles
- EP ID EP654381
- DOI 10.24297/jab.v4i1.1940
- Views 169
- Downloads 0
SEASONAL VARIATION OF THE PHYSICO-CHEMISTRY QUALITY OF A TRIBUTARY OF THE BONNY ESTUARY, RIVERS STATE, NIGERIA
Okpoka Creek is a tributary of the Upper Bonny Estuary in the Niger Delta, Nigeria. The study evaluated the effects of season (wet and dry) on the physico-chemical characteristics of this creek. Surface water samples wer...
The Curative Effect of Berberine Nanoparticles and Cisplatin Combination Therapies Against Hepatocarcinogenesis-Induced By N-Nitroso-Diethylamine In Male Rat
This study aimed to investigate whether berberine nanoparticles (BBR-NPs) and/or cisplatin supplementation could prevent hepatocarcinogenesis-induced by N-nitroso-diethylamine (DENA) in male rats. Male Wistar albino rats...
Ganoderma lucidum: A Novel Study for Inhibiting Protease Virulence Weapon of Multiple Drug Resistant and Extended Spectrum Beta-Lactamase Pseudomonas aeruginosa Clinical Isolate
Purpose: This study aimed to inhibit protease enzyme which considered one of the most virulence factors produced by multidrug resistant Pseudomonas aeruginosa clinical isolate using Gandoderma lucidium extract. Methodolo...
An Assessment of The Effect of Processing on The Proximate Constituents of Blood Meal Sourced From Cattle And Goat in Zaria, Nigeria
The work reports the proximate and mineral composition of non-steam processed and processed samples of blood meal sourced from cattle and goat (n = 5) in Zaria, Nigeria using standard analytical techniques. The blood sam...
RETICULOCYTE ASSESSMENT AS A BIOMARKER FOR BONE MARROW ERYTHROPOIETIC FUNCTION IN PREGNANT WOMEN ATTENDING ANTENATAL CLINIC IN BENIN CITY, NIGERIA.
A decrease in iron stores in pregnancy is accompanied by a significant rise in the reticulocyte count .This study was aimed at using reticulocyte production index as a biomarker for erythropoietic function in pregnant wo...