ISOLATION, CHARACTERISTICS, AND ANTIOXIDANT ACTIVITY OF LOW MOLECULAR COMPOUNDS OF FRUIT BODIES LACTARIUS PERGAMENUS (Fr.) Fr MUSHROOMS
Journal Title: JOURNAL OF ADVANCES IN BIOLOGY - Year 2015, Vol 6, Issue 3
Abstract
Methanol and methylene chloride extracts were obtained from fresh and dried fruit bodies of Lactarius pergamenus (Fr.) Fr. and studied by gas-chromatography mass spectrometry (GC/MS), thin-layer chromatography (TLC), and column chromatography on silica gel. Antioxidant activity of individual fractions of mushroom extracts was measured by using DPPH (1,1-diphenyl-2-picrylhydrazyl). Stearic acid was removed from the extracts by their freezing, and phthalic acid esters, sesquiterpene compounds, long chain fatty acids, and their derivatives were found after that GC/MS analysis showed that the most abundant antioxidant compounds in methylene chloride extracts are: velleral dialdehyde, azulene sesquiterpenes, leaf and pelargonic aldehydes, and oleic acid amide. The velleral is known to provide a pungent taste of Lactarius sp. mushrooms. Thus, the antioxidant properties of methanol and methylene chloride extracts of mushrooms of Lactarius genus are not caused by the presence of phenols, flavonoids, β-carotenes, and tocopherols, as it was suggested in other studies. High level of phthalates that was discovered in these mushrooms, probably, is play an important role in the mechanisms of defence of mushrooms from insects. The obtained data are important for controlling chemical composition during isolation of pharmacologically active substances, and for preparation of antifungal ointments, in particular.
Authors and Affiliations
Antonyuk Volodymir, Mariana V. Tsivinska, Lydia V. Panchak, Rostislav S. Stoika
Effect of cis-tetrahydro-B-carboline-1(m-nitrophenyl)-3-carbomethoxy on Leishmania amazonensis
Leishmaniasis is a parasitic disease caused by protozoa of the genus Leishmania, representing a serious public health problem. The worldwide prevalence is estimated to be 12 million cases, with an incidence of 2 million...
ZJXG decoction promoted the fracture healing by reducing the decomposition of BMP-7, IGF-1, bFGF and TGFβ1 in fracture rats
The aim is to investigate the effect of Zhuang Jin Xu Gu (ZJXG) decoction on serum levels of bone morphogenetic protein-7 (BMP-7), insulin-like growth factor-1 (IGF-1), basic fibroblast growth factor (bFGF) and transform...
Augmentation of drought stress tolerance of two wheat cultivars through exogenous spraying of silicon
Two wheat cultivars cv. Sohag 3 (durum wheat) and cv. Seds 12 (bread wheat) were tested to assess their physiological behavior under different water regimes (100%, 70%, 50% and 30% FC) and their responsiveness to foliar...
Comparative study of some parameters biological of Bactrocera dorsalis and Ceratitis cosyra (Diptera :Tephritidae) pests of mango (Mangifera indica) in Co´te d Ivoire
Bactrocera dorsalis and Ceratitis cosyra are the major pests of mango (Mangifera indica), in Cote d'Ivoire. The study of biological parameters of these pests has revealed that the development of B. dorsalis is higher tha...
In vivo and in vitroevaluation of the Acrocomia aculeatapulp oil - lipid profile and oxidative stress
Acrocomia aculeata is a plant species with high levels of antioxidant compounds, rich in carotenoids with high bioavailability and monounsaturated fatty acids. This study was designed to investigate the biological effect...