ANTIOXIDANT, ANTHELMINTIC AND ENZYME INHIBITORY POTENTIAL OF STREPTOMYCES VARIABILIS STRAIN PO-178 ISOLATED FROM WESTERN GHAT SOIL OF AGUMBE, KARNATAKA, INDIA
Journal Title: Journal of Biological and Scientific Opinion - Year 2014, Vol 2, Issue 2
Abstract
The present study was carried out with an aim of determining antioxidant, anthelmintic and enzyme (amylase and pancreatic lipase) inhibitory activity of bioactive Streptomyces variabilis strain PO-178 isolated from a rhizosphere soil sample of Agumbe, Western Ghats of Karnataka, India. The strainPO-178 was grown in Starch casein nitrate broth for ten days at 30oC and the culture filtrate was extracted using butanol in separation funnel. Antioxidant potential of butanol extract was tested using four in vitro antioxidant models viz., DPPH radical scavenging, ABTS radical scavenging, metal chelating and Ferric reducing assay. The extract exhibited moderate antioxidant activity in all assays performed when compared with reference antioxidants. Anthelmintic activity of extract was screened using adult Indian earthworm. The extract caused paralysis and mortality of worms in a dose dependent manner. Enzyme inhibitory activity of butanol extract was determined against amylase and pancreatic lipase. The extract was found to exhibit dose dependent pancreatic lipase inhibitory and amylase inhibitory activity. Further studies on isolation and characterization of biologically active principles from butanol extract of strain PO-178 and their bioactivity determination are under progress.
Authors and Affiliations
Prashith Kekuda , Onkarappa R
Keywords
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- EP ID EP136633
- DOI 10.7897/2321-6328.02239
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