ANTIOXIDANT DEFENSE ENZYMES ACTIVITY IN HYPOBARIC HYPOXIA SUSCEPTIBLE AND TOLERANT SPRAGUE DAWLEY RATS
Journal Title: Int J of Pharm Res & Analy - Year 2014, Vol 4, Issue 8
Abstract
Antioxidant enzymes are known defense molecules, which eliminate reactive oxygen species (ROS) and free radicals that damage useful enzymes in the cell under various stress conditions. Significant efforts have been made to explore these antioxidant enzymes as a potential candidate for the diagnosis of certain diseases. Hypobaric hypoxia is a condition of oxidative stress leading to various diseases through the involvement of free radicals. In the present study, we have analyzed the expression of antioxidant enzymes in the plasma of male albino rats (185 ± 10 g) that were segregated into hypobaric-hypoxia susceptible (HHS) and tolerant (HHT) on the basis of their ‘gasping time’. After one week of normalization, both HHS and HHT rats were exposed to 30,000 ft for 1 h and sacrificed to collect the blood sample. Catalase (CAT) and peroxidase showed 3.9 and 2 fold increased enzymatic activity, respectively in HHT rats as compared to HHS rats. There was no significant difference in superoxide dismutase (SOD) activity in HHT when compared with HHS rats. Differential activity of CAT and peroxidase in HHS and HHT rats under hypoxia stress was also confirmed by zymography. Thus differential activity of these antioxidant enzymes in HHS and HHT may have a regulatory role under hypobaric hypoxic conditions.
Authors and Affiliations
Santosh Kumar1 , Priyanka Sharma1 , Anju Bansal2 , P. C. Sharma1 , K. K. Aggarwal1
Keywords
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