ANTIOXIDANT, ENZYME INHIBITORY AND ANTIFUNGAL ACTIVITIES OF ACTINOMYCETES ISOLATED FROM CURCUMA LONGA RHIZOSPHERE
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2016, Vol 8, Issue 2
Abstract
Objective: To screen the actinobacterial strains associated with root rhizosphere of Curcuma longa for antioxidant, enzyme inhibitory and antifungal potential and to identify the potent strain TRA-1.Methods: The strains were isolated on starch casein agar (SCA). The antioxidant, enzyme inhibitory and antifungal activities were studied using standard methods. Identification of active strain TRA-1 was carried out by studying morphological and cultural characteristics and by employing 16S rRNA sequencing.Results: The detected phenolic content and total antioxidant activities were in the range of 1µg/ml to 80 µg/ml and 0.198 to 1.088 each. Diphenyl picrylhydrazyl (DPPH) and hydroxyl radicals scavenging activity and capacity to chelate metal ion and reduce ferric iron were found in 11, 14, 6 and 12 strains respectively. The activities of α-amylase and α-glucosidase were inhibited by crude extracts of twelve and three isolates and the extracts of ten isolates inhibited lipase activity. Nine isolates inhibited the growth of one or more test fungal pathogens. All test pathogens were sensitive to TRA-1. The active strain TRA-1 was identified as Streptomyces albus. The active metabolite of TRA-1 showed 41.43% DPPH scavenging activity, 54.48% and 34.46% respective inhibition in α-amylase and lipase activity and was highly active against C. neoformans (35 mm) followed by A. niger (18 mm).Conclusion: The results of the study showed remarkable antioxidant, enzyme inhibitory and antifungal potential of isolated strains. The potent strain Streptomyces albus TRA-1 can be used as a promising source for isolation of pharmaceutically important bioactive compounds.Keywords: Antioxidant, Inhibition, Enzyme, Antifungal, Streptomyces albus.
Authors and Affiliations
Hemlata Bhosale, Priti Bismile, Tukaram Kadam, Uzma Shaheen
Keywords
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