Antioxidant enzymes preserving coffee quality in refrigerate environment
Journal Title: Biotecnología Vegetal - Year 2018, Vol 18, Issue 3
Abstract
Post-harvest phases may cause biochemical and physiological changes with direct effects on coffee (Coffea sp.) grains quality during its storage. The aim of this study was to determine the relevance of antioxidant isoenzyme expression on the coffee grains quality undergoing different types of processing and storage conditions. Coffea arabica L. ripe fruits was harvested, part of them was dry processed and another part was wet processing. Immediately, the coffee was dried. After drying, some of the grains were hulled and the other part was maintained unhulled. After that, the grains were stored under refrigerate controlled conditions (10 oC and 50% relative humidity) or at 25 oC without relative humidity control for a period of 12 months. Enzymatic expression of catalase, esterase, peroxidase, and alcohol dehydrogenase enzymes was evaluated in the coffee grains in these storage conditions by electrophoretic analysis. The results were compared to the sensorial and physiological profiles of the samples. It was found that expression of enzymes of the antioxidative process is associated with changes in the quality of coffee grains. Natural coffee obtained by dry processing is more sensitive to biochemical changes than wet-processed coffee. A refrigerated environment has a beneficial effect for preserving the coffee grains showed by higher expression of catalase, peroxidase, and alcohol dehydrogenase enzymes after six months of storage in these conditions. The protection of the endosperm by the presence of the pericarp in natural coffee or the endocarp in fully-washed coffee is beneficial to maintenance of quality and is related to expression of antioxidant enzymes.
Authors and Affiliations
Giselle Figueiredo de Abreu , Sttela Dellyzete Veiga Franco da Rosa , Marcelo Ribeiro Malta , Aline da Consolação Sampaio Clemente , Amanda Lima Vilela , Rucyan Walace Pereira
Keywords
Related Articles
- EP ID EP682976
- DOI -
- Views 148
- Downloads 0
Field evaluation of regenerated plants by somatic embryogenesis from shoots apexes from axilary buds in cv.‘Navolean’ (Musa spp., AAB)
The use of shoots apexes from axilary buds for callus induction with embryogenic structures in plantain ‘Navolean’ (Group AAB) permitted to develop a plant regeneration method through out somatic embryogenesis. In order...
In vitro propagation of Pinus cubensis Griseb plants
Micropropagation of plants from the genus Pinus has been done in several species. However, micropropagation of Pinus cubensis Griseb has not been reported. This species has a great economical importance. Then, the aim...
Morphological characteristics of Pinus caribaea in vitro plants var. caribaea influenced by the use of sucrose in the multiplication phase
The genus Pinus has been classified as recalcitrant in relation to in vitro root formation. This work was carried out to evaluate the influence of sucrose on the morphologic characteristics of Pinus caribaea var. cariba...
Assessment on cytotoxic and mutagenic potency of Gamma rays and EMS in Vigna mungo L. Hepper
To determine the mutagen agent potency and deduce an optimum dose, cytological analysis for induced chromosomal aberrations is considered as an accurate index in mutation breeding. Therefore, the present investigation...
In vitro multiplication of ginger Zingiber officinale Roscoe, cv. ‘Grand Caiman’ in Temporary Immersion System
Ginger (Zingiber officinale Roscoe) is a plant of great interest for its properties and uses. Although it has been in vitro propagated in semi-solid culture media, the use of Temporary Immersion Systems (TIS) could be an...