Antioxidative effect of peroxiredoxin-3 in the rat myocardium exposed to renal ischemia-reperfusion injury
Journal Title: TMR Integrative Medicine - Year 2020, Vol 4, Issue 10
Abstract
Background: Peroxiredoxin-3 (Prx-3) is known to be involved in the clearance of cellular hydrogen peroxide and plays an important role in protecting the myocardial tissue against damage of oxidative stress. However, the role of Prx-3 in protecting the myocardial tissue against renal ischemia/reperfusion (I/R)-induced myocardial injury (RI/RMI) remains unknown. We aimed to examine the antioxidative effect of Prx-3 using a rat RI/RMI model in an attempt to find a new approach to the prevention and treatment of myocardial injury induced by renal I/R. Methods: A RI/RMI rat model was established to detect the renal histopathology and renal functions by hematoxylin-eosin staining staining and commercially available assay kits. The myocardial antioxidant activity and the expressions of mRNA and protein of Prx-3 in the myocardial tissue were measured by real-time polymerase chain reaction and Western blotting assay, respectively. Results: Compared with the normal and control groups, the serum level of blood urea nitrogen and serum creatinine was significantly increased in the I/R group (P < 0.05). Histopathological changes in the kidneys were also more obvious, including glomerular cystic and renal interstitial hemorrhage, renal tubular epithelial cell edema, and tubular type formation. The mRNA and protein expressions of Prx-3 were increased significantly in the RI/MRI rat model as compared with those in the normal and control groups. Conclusion: Oxidative damage occurred in the remote myocardium after renal I/R and Prx-3 was able to enhance the self-regulatory ability of the myocardium against damage from oxidative stress after RI/MRI.
Authors and Affiliations
Xue-Bo Li, Xin Jin, Long Xu, Bo Hu, Guang-Tao Xu, De-Qing Chen
Keywords
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- EP ID EP682949
- DOI 10.12032/TMRIM202004010
- Views 251
- Downloads 0
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