ANTIVIRAL ACTIVITY AND ITS PROSPECTIVE MECHANISM OF ACTION ON NEWCASTLE DISEASE VIRUS USING CRUDE EXTRACT OF FOUR MEDICINAL PLANTS
Journal Title: World Journal of Pharmaceutical Research - Year 2018, Vol 7, Issue 11
Abstract
Viral problems have been in focused of the scientists due to their high metabolic rate, drug resistance and unique nature of pathological mechanism. The failure of novel synthetic allopathic antiviral drugs impels the scientists to investigate other sources of alternative antiviral agents. Herbal extracts has various inhibitory effects against avian viruses particularly Newcastle disease virus (NDV). The current study was under taken to evaluate in-vitro NDV potential of crude extracts of different medicinal plants by means of Haemagglutination (HA) titer in vero cell line culture. To study the potential NDV activity, Vero cell line were treated with different doses (25μl, 50μl, 75μl and 100μl) of solvent free crude extracts of G. glabra, P. emblica, A. sativum and A. indica and interacted with 1000 TCID50 of the lasota virus during infection at different time periods. Minimum dose (25μg/ml) of crude ethanolic extract of A. indica when inoculated on to vero cell line before NDV exposure showed significantly higher (48 HA titer) anti-NDV response as compare to A. sativum (64 HA), but significantly lower than P. emblica (20 HA titer). Similarly, minimum dose (25μg/ml) of crude ethanolic extract of P. emblica after one hour post NDV exposure showed significantly higher (Mean NDV HA Titer 24) anti-NDV response as compare to A. indica (48 HA Titer) and glycyrrhiza, but similar to A. sativum (48 HA). Whereas, maximum dose (100μg/ml) of P. emblica showed significantly similar antiviral (3HA) response on vero cell infected NDV when treated one hour before with A. indica, A. sativum (3 HA), but significantly higher than G. glabra (128 HA) in terms of mean HA titer. Maximum tested dose (100μg/ml) of P. emblica post one hour NDV exposure showed significantly similar antiviral (2.5 HA) response on vero cell to A. indica (6HA) and A. sativum (4 HA), but significantly higher than G. glabra (192). However, either of the selected plants did not show any cell toxicity by means of cytopathic effect (CPE) and Haemagglutination activity alone or synergistically even at 100μg/ml. It is evaluated that simultaneous treatment (25μg/ml) with crude extract of A. sativum to expose NDV (EID50=105) in 90% saturated vero cell line showed substianal antiNDV activity.
Authors and Affiliations
Muhammad Danish Mehmood
Keywords
Related Articles
- EP ID EP627600
- DOI -
- Views 105
- Downloads 0
AN ASSISTING SOFTWARES USING DRUG DESIGN
Drug designing software’s has potential role to design novel proteins or drugs in biotechnology or pharmaceutical field. The drug designing software’s are used to analyze molecular modeling of gene, gene expression, gene...
EVALUATION OF TOXICOLOGICAL PROFILE OF KOJU®- A NIGERIAN POLYHERBAL FORMULATION, IN WISTAR RATS
Increased morbidity and mortality associated with the use of herbs/ herbal products has raised universal attention in the last few years, making the safety and toxicity evaluations of these preparations imperative. This...
IS DIABETES AN AUTONOMIC DISORDER?
Dysfunction of the cardiac Autonomic Nervous System (ANS) in the diabetic heart is involved in cardiovascular morbidity and mortality, and in the development of degenerative complications of diabetes. Objectives of the s...
AN INVESTIGATION IN TO THE TRANSVERSE STRENGTH OF LIGHT CURE DENTURE BASE MATERIAL REINFORCED WITH GLASS FIBER
Background: Visible light cured acrylic resin denture materials are one of the developed polymeric acrylic denture base and are manufactured in the form of sheets and rapes, and also in powder and liquid system, they can...
ENHANCEMENT OF SOLUBILITY OF POORLY SOLUBLE DRUG BY MIXED SOLVENCY CONCEPT
In the present study, practically water insoluble drug, meloxicam was tried to be solubilized by employing the combination of physiologically compatible menthol: thymol mixture in the form of their eutectic mixture to ma...