Application of analyte-mediated enzyme-activating signal amplification system in elisa for determining low concentrations of bioanalytes
Journal Title: Problems of Environmental Biotechnology - Year 2015, Vol 1, Issue 1
Abstract
In this work were conducted development and characterization of enzyme immunoassay (ELISA) to determine total IgE-antibodies using tyramine-biotin reagent (TBR). It was shown that the use of ELISA modifications with avidin-biotin amplification (ABA) and TBR significantly extend application analysis dose human IgE below 50 IU/ml. For the purposes of clinical diagnosis was the most appropriate ELISA with ABA, however, when there is the need to define micro-concentrations of analyte (10-10 g), the only possible option is ELISA with TBR. When used ELISA reagent concentration dynamic range for all modifications made three orders.
Authors and Affiliations
О. Ю. Галкін, Ю. В. Горшунов
Keywords
Related Articles
- EP ID EP31556
- DOI -
- Views 469
- Downloads 2
Different types of biosensors and their features
Biosensor technology is a powerful alternative to conventional techniques. Biosensors are analytical devices that respond selectively to analytes in an appropriate sample and convert their concentration into an electrica...
Cotoneaster (Cotoneaster Medik.) – new object of biotechnology
The influence of the water-salt extracts of Cotoneaster buds and foliages Series Adpressi, Melanocarpi, Salicifoli on functional activity of the phagocyte were studied by us. It is recommended to use their foliages as a...
Investigation of expression of high-molecular substances as the consequence of exposure to laser light on the organism of earth worm Eisenia veneta
The effect of laser light on the content of fractions of soluble and a membrane protein in tissues of worms of parental forms and generation F1 of the species Eisenia veneta was estimated. Studies have shown changes in...
Future use plant material in brewing
An important characteristic of beer is balanced organoleptic and physicochemical parameters, their harmonious combination, but the beer is organic product with continued biochemical processes that contribute to the dete...
Study experience of using plant materials infected by fungi as substrate for microbial fuel cell
Article presents the results of experimental investigation applicability of plant fallen materials contaminated by powdery mildew fungus E. flexuosa as substrate in microbial fuel cell (MFC). MCFs were presented in form...