Application of analyte-mediated enzyme-activating signal amplification system in elisa for determining low concentrations of bioanalytes

Journal Title: Problems of Environmental Biotechnology - Year 2015, Vol 1, Issue 1


In this work were conducted development and characterization of enzyme immunoassay (ELISA) to determine total IgE-antibodies using tyramine-biotin reagent (TBR). It was shown that the use of ELISA modifications with avidin-biotin amplification (ABA) and TBR significantly extend application analysis dose human IgE below 50 IU/ml. For the purposes of clinical diagnosis was the most appropriate ELISA with ABA, however, when there is the need to define micro-concentrations of analyte (10-10 g), the only possible option is ELISA with TBR. When used ELISA reagent concentration dynamic range for all modifications made three orders.

Authors and Affiliations

О. Ю. Галкін, Ю. В. Горшунов


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  • EP ID EP31556
  • DOI -
  • Views 354
  • Downloads 2

How To Cite

О. Ю. Галкін, Ю. В. Горшунов (2015). Application of analyte-mediated enzyme-activating signal amplification system in elisa for determining low concentrations of bioanalytes. Problems of Environmental Biotechnology, 1(1), -.