Application of analyte-mediated enzyme-activating signal amplification system in elisa for determining low concentrations of bioanalytes
Journal Title: Problems of Environmental Biotechnology - Year 2015, Vol 1, Issue 1
Abstract
In this work were conducted development and characterization of enzyme immunoassay (ELISA) to determine total IgE-antibodies using tyramine-biotin reagent (TBR). It was shown that the use of ELISA modifications with avidin-biotin amplification (ABA) and TBR significantly extend application analysis dose human IgE below 50 IU/ml. For the purposes of clinical diagnosis was the most appropriate ELISA with ABA, however, when there is the need to define micro-concentrations of analyte (10-10 g), the only possible option is ELISA with TBR. When used ELISA reagent concentration dynamic range for all modifications made three orders.
Authors and Affiliations
О. Ю. Галкін, Ю. В. Горшунов
Keywords
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