Application of Derivative Spectrophotometer for Analysis of Chloroquine Phosphate Dosage Forms
Journal Title: Journal of Pharmaceutical Research International - Year 2016, Vol 12, Issue 2
Abstract
Afirst derivative spectrophotometric method was developed for the determination of chloroquine phosphate in formulations. The aim of the present work is to develop and optimize a derivative spectrophotometric method for the analysis of chloroquine phosphate substance and to study of the expected interference of pharmaceutical excipent used in chloroquine phosphate formulations on the developed method, to employ the developed method for analysis of chloroquine phosphate in pharmaceutical dosage forms, and to compare between the developed method and the official methods for analysis of chloroquine phosphate formulations. The zero order absorption and first derivative spectra were measured for chloroquine phosphate working standard and formulation using UV spectrophotometer. The derivative procedure was based on the linear relationship between the concentration of chloroquine phosphate and first order derivative amplitude at 225 nm, 239 nm, 260 nm, and 349 nm. The first derivative spectra were developed between 200 nm- 400 nm. The developed method was compared with high performance liquid chromatography (HPLC) and the official non-aqueous titration methods (BP and USP). The results obtained showed good linearity and selectivity in the concentration range 2.5 -50 µg/ml. The developed method was successfully applied in the quantitative assay of commercial tablets, injections and syrup formulations. The method was simple, rapid and suitable for quality control application.
Authors and Affiliations
Siham Abdallah, E. E. Kamal, M. E. M. Hagga, Ishraqa Mohamed
Keywords
Related Articles
- EP ID EP342126
- DOI 10.9734/BJPR/2016/23913
- Views 109
- Downloads 0
HPTLC Methods for Determination of Gemifloxacin Mesylate in Rabbit Plasma
Aim: A new, simple, rapid, very sensitive and accurate high performance thin-layer chromatographic (HPTLC) method has been developed and validated for estimation of Gemifloxacin in rabbit plasma. Study Design: Validation...
Comparative Fatty Acids Profiling and Antioxidant Potential of Pawpaw and Watermelon Seed Oils
Seed oils of watermelon, unripe and ripe pawpaw were extracted and analyzed for their fatty acid composition using GC/EIMS, phenolic content and antioxidant potential using free radical scavenging effect on 2, 2-diphenyl...
Effects of Fermented Soya Bean Supplements on Thyroid Profile and Serum Electrolytes of High Fat Diet-induced Type 2 Diabetes Mellitus in Rabbits
The aim of this study was to evaluate the effects of fermented soya bean supplements on thyroid profile and serum electrolytes of high fat diet-induced type 2 diabetes in rabbits. Twenty rabbits weighing between 1 kg –...
Phytochemistry, Phenolic Contents and Free Radical Scavenging of Bauhinia thoningii kurtz and Ricinus communis L Extracts as Possible Contribution to Medicinal Effects
Aims: Current research places premium on isolation bioactive constituents and antioxidants activities of plants. However, the chemical constituents and antioxidant potential of many local medicinal plants are still unkn...
Study of Preventive Effectiveness of Nigella sativa L. against Rat kidney Stones, Generated by Ethylene Glycol
Aims: The objective of this research is to study the preventive effectiveness of Nigella sativa L. against rat kidney stones, generated by Ethylene Glycol. Methodology: In this study, 40 Wistar rats were categorized r...