Application of ISSR molecular markers in genetic diversity of three Trifolium species
Journal Title: Journal of Biodiversity and Environmental Sciences (JBES) - Year 2013, Vol 3, Issue 12
Abstract
Genetic variation for of 14 accessions of three Trifolium species of evaluated using 10 ISSR primers. In total 75 bands (loci) were polymorph in accessions studied. Primer IS12 with 11 bands had the highest and primer IS15 with 4 bands had the lowest number of bands. The primers of IS12, IS13 and IS10 considering polymorphism information content (PIC) were useful for polymorphism study in Trifolium species genus in the future researches. AMOVA revealed that 29% of the total variance was due to differences between species and 71% was due to differences within species. T.prateuse specie with the Shannon's information index (I) and Nei's gene diversity (He) had the highest variety between reviewed species, and T.hybridum specie had the lowest variety. In addition the species T.prateuse had the highest similarity with T.fragiferum. These results were confirmed by cluster analysis and principal coordinate analysis of species. Cluster analysis for reviewing accession, classified them in three groups. Cluster analysis and Scatter plot based on first and second axis from principal coordinate analysis for accessions, showed that the primers ISSR could clearly separate species and accessions of each species were placed to each other.
Authors and Affiliations
Parisa Aryanegad, Ezatollah Farshadfar, Hooshmand Safari, Hooman Shirvani
Keywords
Related Articles
- EP ID EP37443
- DOI -
- Views 144
- Downloads 0
Floristic composition and life form classes of district Shangla, Khyber Pakhtunkhwa, Pakistan
The present study was conducted in order to explore the floristic composition and life form classes of district Shangla, Khyber Pakhtunkhwa Pakistan. Floristic survey of district Shangla was conducted from 15 March 2011...
Phylogenetic relationships between Trigonella species (Bucerates section) using ITS markers and morphological traits
Trigonella L. (Fabaceae) includes about 135 species worldwide and most of the species are distributed in the dry regions around Mediterranean. Phylogenetic relationships of 18 species of medicagoid and one representativ...
Temporal Trend Calculation of DI (Drought Index) and comparison of two methods of IDW and KRG as important Spatial Analysis tools
Drought is one of the main natural causes of agricultural, economic and environmental damage. The objective of this study is to provide a comparative spatial analysis by using IDW and KRG methods in Taleghan Watershed i...
Heavy metals bioaccumulation in muscle tissues of Catla catla, Labeo rohita and Cirrhinus mrigala exposed to Chakbandi drain water concentrations in glass aquaria
This project was planned to study the heavy metals bioaccumulation in muscle tissues of Catla catla, Labeo rohita and Cirrhinus mrigala exposed to Chakbandi drain water concentrations in glass aquaria. In this study, Cha...
The investigation and study of faune of the genus Chersotis Boisduval, 1840 in Iran
In this paper, the distribution of the Chersotis species from Iran are listed. Original data and published data by the author have been used as well.