Application of peptide antibodies to studies on the immunodominant conformation dependent epitopes of human thyroid peroxidase
Journal Title: Postępy Nauk Medycznych - Year 2011, Vol 24, Issue 11
Abstract
The discontinuous immunodominant regions (IDRs) recognized by autoantibodies directed to thyroid peroxidase (TPO) have not been unequivocally localized. We have explored the location of the IDRs by generation rabbit anti-TPO peptide antibodies and competition experiments with monoclonal antibodies (moabs) specific for those IDRs. Previously we suggested the localization of IDR-A and IDR-B and here we tested the validity of our conclusions. The specificity, reactivity with native TPO, cross reactivity with homologous proteins, the effect of steric hindrance, and the possibility of conformational changes induced in TPO by peptide antibody binding have been explored. The inhibition of IDR-B specific moabs and autoantibodies binding to TPO approaching 90% by peptide antibodies or their mixture call for an explanation and we think that at least part of those peptide amino acid sequences could be involved in building the IDR-A and IDR-B regions.
Authors and Affiliations
Marlena Godlewska, Alicja Bauer, Barbara Czarnocka, Andrzej Gardas
Keywords
Related Articles
- EP ID EP54073
- DOI -
- Views 119
- Downloads 0
Resekcja gruczolakoraka przełyku i wpustu techniką przezrozworową z użyciem toru wizyjnego laparoskopu
Wstęp. Niski odsetek 5-letniego przeżycia chorych na raka gruczołowego przełyku i wpustu jest przyczyną poszukiwania optymalnych technik jego leczenia. Jedną z nich jest przezrozworowa resekcja przełyku przy użyciu toru...
Przewlekła obturacyjna choroba płuc (POChP) w wieku podeszłym
POChP jest poważnym problemem zdrowia publicznego, które dotyka znaczną część populacji osób starszych. Częstość występowania POChP u osób po 65. r.ż. szacuje się na 50% w porównaniu z 20% w wieku 40 lat. Objawy POChP u...
Transvaginal tubal infertility treatment – preliminary study
Introduction. Fallopian tubes abnormalities represent a significant percentage of the causes of female infertility. In a study of 3424 hysterosalpingogram, 15.2% unilateral and 12.2% bilateral obstructions was detected....
Aging – what’s new?
Skin aging is a natural process, which consists in reducing the biological activity of body’s cells, slowing down regenerative processes and loss of adaptability. The decisive role is played by genetic factors and associ...
An assessment of the frequency of lymphocytes with IL-4 intracellular expression and IL-4 concentration in the plasma of patients with chronic lymphocytic leukemia depending on the occurrence of Epstein-Barr virus DNA in peripheral blood mononuclear cells
Introduction. Epstein-Barr virus (EBV), also referred to as human herpesvirus 4 (HHV-4), is the first identified human virus with documented involvement in carcinogenesis. Chronic lymphocytic leukemia (CLL) is a neoplast...